Immobilization in polyvinyl alcohol hydrogel enhances yeast storage stability and reusability of recombinant laccase-producing S. cerevisiae
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To improve the storage stability and reusability of various yeast strains and species by immobilization in polyvinyl alcohol (PVA) hydrogel particles.
Debaryomyces hansenii, Pichia sorbitophila, Saccharomyces cerevisiae, Yarrowia lipolytica, and Zygosaccharomyces rouxii were immobilized in PVA particles using LentiKats technology and stored in sterile water at 4 °C. The immobilization improved the survival of all species; however, the highest storage stability was achieved for S. cerevisiae and Y. lipolytica which survived more than 1 year, in contrast to free cells that survived for only 3 months. Tests of the reusability of immobilized recombinant laccase-secreting S. cerevisiae revealed that the cells were suitable for repetitive use (55 cycles during 15 months) even after storage in water at 4 °C for 9 months. A suitable method for killing immobilized laccase-secreting cells without affecting the produced enzyme activity was also developed.
The immobilization of yeasts in PVA hydrogel enables long-term, cheap storage with very good cell viability and productivity, thus becoming a promising approach for industrial applications.
KeywordsImmobilization Laccase LentiKats Polyvinyl alcohol hydrogel Reusability Storage stability Yeasts
We thank Dr. Radek Stloukal from LentiKat´s a.s., Stráž pod Ralskem, Czech Republic for sharing the know-how on LentiKats technology and necessary material for the immobilization. S. cerevisiae T73 was a kind gift from Prof. Amparo Querol, IATA-CSIC (Valencia, Spain) and S. cerevisiae FW was obtained from Dr. M. Opekarová, Institute of Microbiology, Czech Academy of Sciences. This work was supported by grant TA01011461 from the Technological Agency of the Czech Republic.
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