Biotechnology Letters

, Volume 40, Issue 2, pp 335–341 | Cite as

Species of family Promicromonosporaceae and family Cellulomonadeceae that produce cellulosome-like multiprotein complexes

  • Wei Wang
  • Yang Yu
  • Tong-Yi Dou
  • Jia-Yue Wang
  • Chenggong Sun
Original Research Paper
  • 97 Downloads

Abstract

Objectives

To screen the phylogenetically-nearest members of Cellulosimicrobium cellulans for the production of cellulosome-like multienzyme complexes and extracellular β-xylosidase activity against 7-xylosyltaxanes and to get corresponding molecular insights.

Results

Cellulosimicrobium (family Promicromonosporaceae) and all genera of the family Cellulomonadeceaec produced both cellulosome-like multienzyme complexes and extracellular β-xylosidase activity, while the other genera of the family Promicromonosporaceae did not. Multiple sequence alignments further indicated that hypothetic protein M768_06655 might be a possible key subunit.

Conclusion

This is the first report that many actinobacteria species can produce cellulosome-like multienzyme complexes. The production of cellulosome-like complexes and the extracellular β-xylosidase activity against 7-xylosyltaxanes might be used to differentiate the genus Cellulosimicrobium from other genera of the family Promicromonosporaceae.

Keywords

Cellulomonadeceae Cellulosome Multienzyme complexes Promicromonosporaceae β-Xylosidase 7-Xylosyltaxanes 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31600641) and the Fundamental Research Funds for the Central Universities (No. DUT16RC(3)016).

Supporting Information

Supplementary Fig. 1—Multiple sequence alignment results (related to Table 2).

Supplementary material

10529_2017_2469_MOESM1_ESM.docx (23 kb)
Supplementary material 1 (DOCX 22 kb)

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  1. 1.The Second Affiliated Hospital of Dalian Medical UniversityDalianChina
  2. 2.School of Life Science and MedicineDalian University of TechnologyPanjinChina
  3. 3.Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalianChina

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