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Species of family Promicromonosporaceae and family Cellulomonadeceae that produce cellulosome-like multiprotein complexes

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Abstract

Objectives

To screen the phylogenetically-nearest members of Cellulosimicrobium cellulans for the production of cellulosome-like multienzyme complexes and extracellular β-xylosidase activity against 7-xylosyltaxanes and to get corresponding molecular insights.

Results

Cellulosimicrobium (family Promicromonosporaceae) and all genera of the family Cellulomonadeceaec produced both cellulosome-like multienzyme complexes and extracellular β-xylosidase activity, while the other genera of the family Promicromonosporaceae did not. Multiple sequence alignments further indicated that hypothetic protein M768_06655 might be a possible key subunit.

Conclusion

This is the first report that many actinobacteria species can produce cellulosome-like multienzyme complexes. The production of cellulosome-like complexes and the extracellular β-xylosidase activity against 7-xylosyltaxanes might be used to differentiate the genus Cellulosimicrobium from other genera of the family Promicromonosporaceae.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31600641) and the Fundamental Research Funds for the Central Universities (No. DUT16RC(3)016).

Supporting Information

Supplementary Fig. 1—Multiple sequence alignment results (related to Table 2).

Author information

Authors and Affiliations

  1. The Second Affiliated Hospital of Dalian Medical University, Dalian, 116011, China

    Wei Wang, Yang Yu & Chenggong Sun

  2. School of Life Science and Medicine, Dalian University of Technology, Dagong Road No. 2, LiaoDongWan New District, Panjin, 124221, China

    Tong-Yi Dou

  3. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China

    Jia-Yue Wang

Authors
  1. Wei Wang
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  2. Yang Yu
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  3. Tong-Yi Dou
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  4. Jia-Yue Wang
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  5. Chenggong Sun
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Corresponding author

Correspondence to Tong-Yi Dou.

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Wang, W., Yu, Y., Dou, TY. et al. Species of family Promicromonosporaceae and family Cellulomonadeceae that produce cellulosome-like multiprotein complexes. Biotechnol Lett 40, 335–341 (2018). https://doi.org/10.1007/s10529-017-2469-0

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  • DOI: https://doi.org/10.1007/s10529-017-2469-0

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