Copper oxide nanoparticles induce anticancer activity in A549 lung cancer cells by inhibition of histone deacetylase
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Copper oxide nanoparticles (CuO NPs) promoting anticancer activity may be due to the regulation of various classes of histone deacetylases (HDACs).
Green-synthesized CuO NPs significantly arrested total HDAC level and also suppressed class I, II and IV HDACs mRNA expression in A549 cells. A549 cells treated with CuO NPs downregulated oncogenes and upregulated tumor suppressor protein expression. CuO NPs positively regulated both mitochondrial and death receptor-mediated apoptosis caspase cascade pathway in A549 cells.
Green-synthesized CuO NPs inhibited HDAC and therefore shown apoptosis mediated anticancer activity in A549 lung cancer cell line.
KeywordsAnticancer activity Apoptosis A549 cells Copper oxide nanoparticles Epigenetic Histone deacetylase
We thank the DST-FIST for their infrastructure support to our department. The first author thanks to Dr. K. Jayaraman, Department of Educational Technology, Bharathidasan University, Tiruchirappalli, for the constant encouragement and financial support throughout her career. The authors are grateful to Dr. C. Prahalathan and Dr. A. Antony Joseph Velanganni, Department of Biochemistry, Bharathidasan University, Tiruchirappalli, India for his help with gel documentation and fluorescence studies. Sincere thanks to Dr. S. Sivaramakrishnan, Department of Biotechnology and Genetic Engineering, for his help with gel documentation studies and cell storage.
Supplementary Fig. 1—Field emission-scanning electron microscopic analysis of a CuO NPs b EDAX spectrum (CuO NPs- Copper oxide nanoparticles; EDAX- Energy dispersive X-ray spectroscopy).
Supplementary Table 1—The sequence of the primers used for the RT-PCR (RT-PCR- Reverse transcription PCR).
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Conflict of interest
The authors declare that they have no conflicts of interest concerning this article.