Biotechnology Letters

, Volume 40, Issue 2, pp 249–256 | Cite as

Copper oxide nanoparticles induce anticancer activity in A549 lung cancer cells by inhibition of histone deacetylase

  • Arunachalam Kalaiarasi
  • Renu Sankar
  • Chidambaram Anusha
  • Kandasamy Saravanan
  • Kalyanasundaram Aarthy
  • Selvaraj Karthic
  • Theodore lemuel Mathuram
  • Vilwanathan Ravikumar
Original Research Paper



Copper oxide nanoparticles (CuO NPs) promoting anticancer activity may be due to the regulation of various classes of histone deacetylases (HDACs).


Green-synthesized CuO NPs significantly arrested total HDAC level and also suppressed class I, II and IV HDACs mRNA expression in A549 cells. A549 cells treated with CuO NPs downregulated oncogenes and upregulated tumor suppressor protein expression. CuO NPs positively regulated both mitochondrial and death receptor-mediated apoptosis caspase cascade pathway in A549 cells.


Green-synthesized CuO NPs inhibited HDAC and therefore shown apoptosis mediated anticancer activity in A549 lung cancer cell line.


Anticancer activity Apoptosis A549 cells Copper oxide nanoparticles Epigenetic Histone deacetylase 



We thank the DST-FIST for their infrastructure support to our department. The first author thanks to Dr. K. Jayaraman, Department of Educational Technology, Bharathidasan University, Tiruchirappalli, for the constant encouragement and financial support throughout her career. The authors are grateful to Dr. C. Prahalathan and Dr. A. Antony Joseph Velanganni, Department of Biochemistry, Bharathidasan University, Tiruchirappalli, India for his help with gel documentation and fluorescence studies. Sincere thanks to Dr. S. Sivaramakrishnan, Department of Biotechnology and Genetic Engineering, for his help with gel documentation studies and cell storage.

Supporting information

Supplementary Fig. 1—Field emission-scanning electron microscopic analysis of a CuO NPs b EDAX spectrum (CuO NPs- Copper oxide nanoparticles; EDAX- Energy dispersive X-ray spectroscopy).

Supplementary Table 1—The sequence of the primers used for the RT-PCR (RT-PCR- Reverse transcription PCR).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest concerning this article.

Supplementary material

10529_2017_2463_MOESM1_ESM.docx (718 kb)
Supplementary material 1 (DOCX 718 kb)
10529_2017_2463_MOESM2_ESM.docx (15 kb)
Supplementary material 2 (DOCX 15 kb)


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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  • Arunachalam Kalaiarasi
    • 1
  • Renu Sankar
    • 1
    • 2
  • Chidambaram Anusha
    • 1
  • Kandasamy Saravanan
    • 1
  • Kalyanasundaram Aarthy
    • 1
  • Selvaraj Karthic
    • 3
  • Theodore lemuel Mathuram
    • 4
  • Vilwanathan Ravikumar
    • 1
  1. 1.Department of Biochemistry, School of Life SciencesBharathidasan UniversityTiruchirappalliIndia
  2. 2.Food Animal Health Research Program, Ohio Agricultural Research and Development CenterThe Ohio State UniversityWoosterUSA
  3. 3.Division of Drug Research/Clinical Pharmacology, Department of Medical and Health SciencesLinköping University HospitalLinköpingSweden
  4. 4.Department of Cellular and Molecular BiochemistryFrontier Mediville (A Unit of Frontier Lifeline and Dr. K. M. Cherian Heart Foundation), Affiliated to University of MadrasChennaiIndia

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