Biotechnology Letters

, Volume 39, Issue 5, pp 731–738 | Cite as

Co-cultivation of Chlamydomonas reinhardtii with Azotobacter chroococcum improved H2 production

  • Lili Xu
  • Xianglong Cheng
  • Shuangxiu Wu
  • Quanxi Wang
Original Research Paper

Abstract

Objectives

To improve H2 production, the green algae Chlamydomonas reinhardtii cc849 was co-cultured with Azotobacter chroococcum.

Results

The maximum H2 production of the co-culture was 350% greater than that of the pure algal cultures under optimal H2 production conditions. The maximum growth and the respiratory rate of the co-cultures were about 320 and 300% of the controls, and the dissolved O2 of co-cultures was decreased 74%. Furthermore, the in vitro maximum hydrogenase activity of the co-culture was 250% greater than that of the control, and the in vivo maximum hydrogenase activity of the co-culture was 1.4-fold greater than that of the control. In addition, the maximum starch content of co-culture was 1400% that of the control.

Conclusions

Azotobacter chroococcum improved the H2 production of the co-cultures by decreasing the O2 content and increasing the growth and starch content of the algae and the hydrogenase activity of the co-cultures relative to those of pure algal cultures.

Keywords

Azotobacter chroococcum Chlamydomonas reinhardtii Co-culture Hydrogen production 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (NSFC No. 31600284; No. 31271397).

Supporting information

Supplementary Fig. 1—The micrographs of algae-bacteria aggregate. (a) Pure C. reinhardtii cc849 before fixed it on A. chroococcum; (b) the complex of C. reinhardtii cc849 and A. chroococcum in the initial phase of H2 production; (c) the complex of C. reinhardtii cc849 and A. chroococcum in the rapid growth phases of H2 production; (d) the aggregates of C. reinhardtii cc849 and A. chroococcum in saturated stage of H2 production.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10529_2017_2301_MOESM1_ESM.docx (309 kb)
Supplementary material 1 (DOCX 308 kb)

References

  1. Forestier M, King P, Zhang LP, Posewitz M, Schwarzer S, Happe T, Ghirardi ML (2003) Expression of two [Fe]-hydrogenase in Chlamydomonas reinhardtii under anaerobic conditions. Eur J Biochem 270:2750–2758CrossRefPubMedGoogle Scholar
  2. Gaffron H (1939) Reduction of CO2 with H2 in green plants. Nature 143:204–205CrossRefGoogle Scholar
  3. Gyurjan I, Turtoczky I, Toth G, Gy Paless, Nghia NH (1984) Intercellular symbiosis of nitrogen-fixing bacteria and green alga. Acta Botan Hungar 30:249–256Google Scholar
  4. Harris EH (2009) The Chlamydomonas sourcebook: Introduction to Chlamydomonas and its Laboratory Use, 2nd edn. Academic Press Inc., San DiegoGoogle Scholar
  5. Ietswaartt T, Schneider PJ, Prins RA (1994) Utilization of organic nitrogen sources by two phytoplankton species and a bacterial isolate in pure and mixed cultures. Appl Environ Microbiol 60:1554–1560Google Scholar
  6. Ike A, Toda N, Hirata K, Miyamoto K (1997) Hydrogen photoproduction from CO2-fixing Microalgal Biomass: application of lactic acid fermentation by Lactobacillus amylovorus. J Ferment Bioeng 84:428–433CrossRefGoogle Scholar
  7. Kawaguchi H, Hashimoto K, Hirata K, Miyamoto K (2001a) H2 production from algal biomass by a mixed culture of Rhodobium marinum A-501 and Lactobacillus amylovorus. J Biosci Bioeng 91:277–282CrossRefPubMedGoogle Scholar
  8. Kawaguchi H, Hashimoto K, Hirata K, Miyamoto K (2001b) H2 production from algal biomass by a mixed culture of Rhodobium marinum A-501 and Lactobacillus amylovorus. J Biosci Bioengy 91:277–282CrossRefGoogle Scholar
  9. Kim MS, Baek JS, Yun YS, Sim SJ, Park SH, Kin SC (2006) Hydrogen production from Chlamydomonas reinhardtii biomass using a two-step conversion process: anaerobic conversion and photosynthetic fermentation. Int J Hydrogen Energy 31:812–816CrossRefGoogle Scholar
  10. Krakow JS, Ochoa S (1963) Ribonucleic acid ribonucleic acid nucleotidyl transferase of Azotobacter vinelandii. IV. Purification and properties. Biochem Z 338:796–808PubMedGoogle Scholar
  11. Laurinavichene T, Tolstygina I, Tsygankov A (2004) The effect of light intensity on hydrogen production by sulfur-deprived Chlamydomonas reinhardtii. J Biotechnol 114:143–151CrossRefPubMedGoogle Scholar
  12. Makarova VV, Kosourov S, Krendeleva TE, Semin BK, Kukarskikh GP, Rubin AB, Sayre RT, Ghirardi ML, Seibert M (2007) Photoproduction of hydrogen by sulfur-deprived C. reinhardtii mutants with impaired photosystem II photochemical activity. Photosynth Res 94:79–89CrossRefPubMedGoogle Scholar
  13. Melis A (2007) Photosynthetic H2 metabolism in Chlamydomonas reinahrdtii. Planta 226:1075–1086CrossRefPubMedGoogle Scholar
  14. Melis A, Melnicki MR (2006) Integrated biological hydrogen production. Int J Hydrog Energy 31:1563–1573CrossRefGoogle Scholar
  15. Melis A, Seibert M, Happe T (2000) Sustained photobiological hydrogen gas production upon reversible inactivation of oxygen evolution in the green alga Chlamydomonas reinhardtii. Plant Physiol 122:127–135CrossRefPubMedPubMedCentralGoogle Scholar
  16. Miura Y, Saitoh C, Matsuoka S, Miyamoto K (1992) Stably sustained hydrogen production with high molar yield through a combination of a marine green alga and a photosynthetic bacterium. Biosci Biotechnol Biochem 56:751–754CrossRefPubMedGoogle Scholar
  17. Parmar A, Singh NK, Pandey A, Gnansounou E, Madamwar D (2011) Cyanobacteria and microalgae: a positive prospect for biofuels. Biores Technol 102:10163–10172CrossRefGoogle Scholar
  18. Rubenchik L I (1960) Azotobacter and its uses in agriculture. Akad Nauk Ukrain SSR Insitut Mikrobiol Trans. (Israel Program for Scientific Translations, Jerusalem, 1963.)Google Scholar
  19. Rühle T, Hemschemeier A, Melis A, Happe T (2008) A novel screening protocol for the isolation of hydrogen producing Chlamydomonas reinhardtii strains. BMC Plant Biol 8:107CrossRefPubMedPubMedCentralGoogle Scholar
  20. Walker CC, Yater MG (1978) The hydrogen cycle in nitrogen-fixing Azotobacter chroococcum. Biochimie 60:225–231CrossRefPubMedGoogle Scholar
  21. Wang SP, Cao YC, Li ZJ, Yang YY, Chen SW (2008) The relationship between bacteria and microalgae in water environment and its practical application. South China Fish Sci 4:76–80Google Scholar
  22. Xu LL, Wang QX, Wu SX, Li DZ (2014) Optimization of co-cultivation conditions of transgenic alga hemHc-lbac and Bradyrhizobium japonicum for hydrogen production. Ecol Sci 33:106–112Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Lili Xu
    • 1
  • Xianglong Cheng
    • 1
  • Shuangxiu Wu
    • 2
  • Quanxi Wang
    • 1
  1. 1.Department of Biology, College of Life and Environmental ScienceShanghai Normal UniversityShanghaiPeople’s Republic of China
  2. 2.CAS Key Laboratory of Genome Sciences and Information, Beijing Key Laboratory of Genome and Precision Medicine Technologies, Beijing Institute of GenomicsChinese Academy of SciencesBeijngPeople’s Republic of China

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