Co-cultivation of Chlamydomonas reinhardtii with Azotobacter chroococcum improved H2 production
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To improve H2 production, the green algae Chlamydomonas reinhardtii cc849 was co-cultured with Azotobacter chroococcum.
The maximum H2 production of the co-culture was 350% greater than that of the pure algal cultures under optimal H2 production conditions. The maximum growth and the respiratory rate of the co-cultures were about 320 and 300% of the controls, and the dissolved O2 of co-cultures was decreased 74%. Furthermore, the in vitro maximum hydrogenase activity of the co-culture was 250% greater than that of the control, and the in vivo maximum hydrogenase activity of the co-culture was 1.4-fold greater than that of the control. In addition, the maximum starch content of co-culture was 1400% that of the control.
Azotobacter chroococcum improved the H2 production of the co-cultures by decreasing the O2 content and increasing the growth and starch content of the algae and the hydrogenase activity of the co-cultures relative to those of pure algal cultures.
KeywordsAzotobacter chroococcum Chlamydomonas reinhardtii Co-culture Hydrogen production
This work was supported by the National Natural Science Foundation of China (NSFC No. 31600284; No. 31271397).
Supplementary Fig. 1—The micrographs of algae-bacteria aggregate. (a) Pure C. reinhardtii cc849 before fixed it on A. chroococcum; (b) the complex of C. reinhardtii cc849 and A. chroococcum in the initial phase of H2 production; (c) the complex of C. reinhardtii cc849 and A. chroococcum in the rapid growth phases of H2 production; (d) the aggregates of C. reinhardtii cc849 and A. chroococcum in saturated stage of H2 production.
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Conflict of interest
The authors declare that they have no conflict of interest.
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