High-sensitivity immunochromatographic assay for fumonisin B1 based on indirect antibody labeling
- 370 Downloads
To develop a high-sensitivity immunochromatographic test for fumonisin B1 in plant extracts.
Unlike conventional immunochromatographic tests, this assay is performed in two stages: competitive reaction with free specific antibodies and identifying immune complexes by their interaction with the anti-species antibody-conjugated gold nanoparticles. The use of a new geometry for the test strip membranes and a novel reagent application method ensures the proper order of these stages without additional manipulations. The contact of the ready-to-use test strip with the liquid sample suffices in initiating all stages of the assay and obtaining test results. The developed test was used on corn extracts; its instrumental limit of fumonisin B1 detection was 0.6 ng ml−1 at 15 min of assay duration.
The proposed approach is flexible and can be used for a wide range of low molecular compounds. The use of anti-species antibody-conjugated gold nanoparticles in immunochromatography significantly facilitates the development of test systems by eliminating the need to synthesize and characterize the conjugates with specific antibodies for each new compound to be detected.
KeywordsFumonisin B1 Gold nanoparticles Immunochromatography Indirect labeling Sensitivity enhancement Test strip
This study was financially supported by the Russian Foundation for Basic Research (Grant 15-08-07913).
- Frens G (1973) Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions. Nature 241:20–22Google Scholar
- Malone RJ (2009) Extraction efficiency studies for mycotoxins in naturally contaminated commodities. In: Mycotoxin Prevention and Control in Agricture, vol 1031. ACS Symposium Series, vol 1031. American Chemical Society, pp 223–236Google Scholar
- Tijssen P (1985) Practice and theory of enzyme immunoassays. Elsevier Science, AmsterdamGoogle Scholar