Biotechnology Letters

, Volume 39, Issue 5, pp 739–744 | Cite as

Development of a method for efficient cost-effective screening of Aspergillus niger mutants having increased production of glucoamylase

  • Xudong Zhu
  • Bessembayev Arman
  • Ju Chu
  • Yonghong Wang
  • Yingping Zhuang
Original Research Paper

Abstract

Objectives

To develop an efficient cost-effective screening process to improve production of glucoamylase in Aspergillus niger.

Results

The cultivation of A. niger was achieved with well-dispersed morphology in 48-deep-well microtiter plates, which increased the throughput of the samples compared to traditional flask cultivation. There was a close negative correlation between glucoamylase and its pH of the fermentation broth. A novel high-throughput analysis method using Methyl Orange was developed. When compared to the conventional analysis method using 4-nitrophenyl α-D-glucopyranoside as substrate, a correlation coefficient of 0.96 by statistical analysis was obtained.

Conclusion

Using this novel screening method, we acquired a strain with an activity of 2.2 × 103 U ml−1, a 70% higher yield of glucoamylase than its parent strain.

Keywords

Aspergillus niger Glucoamylase High-throughput screening Microtiter plates 

Notes

Acknowledgements

This work was financially supported by Royal DSM (Delft, The Netherlands) and partially supported by National Basic Research Program (973 Program 2013CB733600), NWO-MoST Joint Program (2013DFG32630).

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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Xudong Zhu
    • 1
  • Bessembayev Arman
    • 1
  • Ju Chu
    • 1
  • Yonghong Wang
    • 1
  • Yingping Zhuang
    • 1
  1. 1.State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghaiPeople’s Republic of China

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