Development of a method for efficient cost-effective screening of Aspergillus niger mutants having increased production of glucoamylase
To develop an efficient cost-effective screening process to improve production of glucoamylase in Aspergillus niger.
The cultivation of A. niger was achieved with well-dispersed morphology in 48-deep-well microtiter plates, which increased the throughput of the samples compared to traditional flask cultivation. There was a close negative correlation between glucoamylase and its pH of the fermentation broth. A novel high-throughput analysis method using Methyl Orange was developed. When compared to the conventional analysis method using 4-nitrophenyl α-D-glucopyranoside as substrate, a correlation coefficient of 0.96 by statistical analysis was obtained.
Using this novel screening method, we acquired a strain with an activity of 2.2 × 103 U ml−1, a 70% higher yield of glucoamylase than its parent strain.
KeywordsAspergillus niger Glucoamylase High-throughput screening Microtiter plates
This work was financially supported by Royal DSM (Delft, The Netherlands) and partially supported by National Basic Research Program (973 Program 2013CB733600), NWO-MoST Joint Program (2013DFG32630).