Biotechnology Letters

, Volume 39, Issue 5, pp 739–744 | Cite as

Development of a method for efficient cost-effective screening of Aspergillus niger mutants having increased production of glucoamylase

  • Xudong Zhu
  • Bessembayev Arman
  • Ju Chu
  • Yonghong Wang
  • Yingping Zhuang
Original Research Paper



To develop an efficient cost-effective screening process to improve production of glucoamylase in Aspergillus niger.


The cultivation of A. niger was achieved with well-dispersed morphology in 48-deep-well microtiter plates, which increased the throughput of the samples compared to traditional flask cultivation. There was a close negative correlation between glucoamylase and its pH of the fermentation broth. A novel high-throughput analysis method using Methyl Orange was developed. When compared to the conventional analysis method using 4-nitrophenyl α-D-glucopyranoside as substrate, a correlation coefficient of 0.96 by statistical analysis was obtained.


Using this novel screening method, we acquired a strain with an activity of 2.2 × 103 U ml−1, a 70% higher yield of glucoamylase than its parent strain.


Aspergillus niger Glucoamylase High-throughput screening Microtiter plates 



This work was financially supported by Royal DSM (Delft, The Netherlands) and partially supported by National Basic Research Program (973 Program 2013CB733600), NWO-MoST Joint Program (2013DFG32630).


  1. An WF, Tolliday N (2010) Cell-based assays for high-throughput screening. Mol Biotechnol 45:180–186CrossRefPubMedGoogle Scholar
  2. Dorr M, Fibinger MP, Last D, Schmidt S, Santos-Aberturas J, Bottcher D, Hummel A, Vickers C, Voss M, Bornscheuer UT (2016) Fully automatized high-throughput enzyme library screening using a robotic platform. Biotechnol Bioeng 113:1421–1432CrossRefPubMedGoogle Scholar
  3. Geddie ML, Rowe LA, Alexander OB, Matsumura I (2004) High throughput microplate screens for directed protein evolution. Protein Eng 388:134–145CrossRefGoogle Scholar
  4. Kumar P, Satyanarayana T (2009) Microbial glucoamylases: characteristics and applications. Crit Rev Biotechnol 29:225–255CrossRefPubMedGoogle Scholar
  5. Liccioli T, Tran TMT, Cozzolino D, Jiranek V, Chambers PJ, Schmidt SA (2011) Microvinification-how small can we go? Appl Microbiol Biotechnol 89:1621–1628CrossRefPubMedGoogle Scholar
  6. Long Q, Liu X, Yang Y, Li L, Harvey L, McNeil B, Bai Z (2014) The development and application of high throughput cultivation technology in bioprocess development. J Biotechnol 192:323–338CrossRefPubMedGoogle Scholar
  7. Lu Y, Wang L, Ma K, Li G, Zhang C, Zhao H, Lai Q, Li H-P, Xing X-H (2011) Characteristics of hydrogen production of an Enterobacter aerogenes mutant generated by a new atmospheric and room temperature plasma (ARTP). Biochem Eng J 55:17–22CrossRefGoogle Scholar
  8. Mayr LM, Bojanic D (2009) Novel trends in high-throughput screening. Curr Opin Pharmacol 9:580–588CrossRefPubMedGoogle Scholar
  9. Persidis A (1998) High-throughput screening. Advances in robotics and miniturization continue to accelerate drug lead identification. Nat Biotechnol 16:488–489CrossRefPubMedGoogle Scholar
  10. Tan J, Chu J, Hao Y, Guo Y, Zhuang Y, Zhang S (2013) High-throughput system for screening of cephalosporin C high-yield strain by 48-deep-well microtiter plates. Appl Biochem Biotechnol 169:1683–1695CrossRefPubMedGoogle Scholar
  11. Tang W, Pan A, Lu H, Xia J, Zhuang Y, Zhang S, Chu J, Noorman H (2015) Improvement of glucoamylase production using axial impellers with low power consumption and homogeneous mass transfer. Biochem Eng J 99:167–176CrossRefGoogle Scholar
  12. Wahler D, Reymond JL (2001) High-throughput screening for biocatalysts. Curr Opin Biotechnol 12:535–544CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Xudong Zhu
    • 1
  • Bessembayev Arman
    • 1
  • Ju Chu
    • 1
  • Yonghong Wang
    • 1
  • Yingping Zhuang
    • 1
  1. 1.State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghaiPeople’s Republic of China

Personalised recommendations