Biotechnology Letters

, Volume 39, Issue 5, pp 701–710 | Cite as

MiRNA-145 suppresses lung adenocarcinoma cell invasion and migration by targeting N-cadherin

  • Dongping Mo
  • Daheng Yang
  • Xuelian Xiao
  • Ruihong Sun
  • Lei Huang
  • Jian Xu
Original Research Paper

Abstract

Objective

To investigate the roles of miR-145 in lung adenocarcinoma (LAC) and to clarify the regulation of N-cadherin by miR-145.

Results

In 57 paired clinical LAC tissues, diminished miR-145 was significantly correlated with the lymph node metastasis and was negatively correlated with N-cadherin mRNA level expression. Wound healing and transwell assays revealed a reduced capability of tumor metastasis induced by miR-145 in LAC. miR-145 negatively regulated the invasion of cell lines through targeting N-cadherin by directly binding to its 3′-untranslated region. Silencing of N-cadherin inhibited invasion and migration of LAC cell lines similar to miR-145 overexpression.

Conclusions

MiR-145 could inhibit invasion and migration of lung adenocarcinoma cell lines by directly targeting N-cadherin.

Keywords

N-Cadherin Invasion Lung adenocarcinoma Migration miR-145 Non-small cell lung cancer 

Notes

Acknowledgements

This study was supported by Key Laboratory for Laboratory Medicine of Jiangsu Province of China (No. XK201114) and Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions. We are grateful to the technical support from National Key Clinical Department of Laboratory Medicine of Jiangsu Province Hospital.

Supporting information

Supplementary Table 1—Target sites and sequences of shRNA plasmids.

Compliance with ethical standards

Conflict of interest

The authors declare no conflicts of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Supplementary material

10529_2017_2290_MOESM1_ESM.doc (30 kb)
Supplementary material 1 (DOC 30 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Dongping Mo
    • 1
  • Daheng Yang
    • 1
  • Xuelian Xiao
    • 1
  • Ruihong Sun
    • 1
  • Lei Huang
    • 1
  • Jian Xu
    • 1
    • 2
  1. 1.Department of Laboratory MedicineThe First Affiliated Hospital of Nanjing Medical UniversityNanjingPeople’s Republic of China
  2. 2.National Key Clinical Department of Laboratory MedicineNanjingChina

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