MiRNA-145 suppresses lung adenocarcinoma cell invasion and migration by targeting N-cadherin
- 260 Downloads
To investigate the roles of miR-145 in lung adenocarcinoma (LAC) and to clarify the regulation of N-cadherin by miR-145.
In 57 paired clinical LAC tissues, diminished miR-145 was significantly correlated with the lymph node metastasis and was negatively correlated with N-cadherin mRNA level expression. Wound healing and transwell assays revealed a reduced capability of tumor metastasis induced by miR-145 in LAC. miR-145 negatively regulated the invasion of cell lines through targeting N-cadherin by directly binding to its 3′-untranslated region. Silencing of N-cadherin inhibited invasion and migration of LAC cell lines similar to miR-145 overexpression.
MiR-145 could inhibit invasion and migration of lung adenocarcinoma cell lines by directly targeting N-cadherin.
KeywordsN-Cadherin Invasion Lung adenocarcinoma Migration miR-145 Non-small cell lung cancer
This study was supported by Key Laboratory for Laboratory Medicine of Jiangsu Province of China (No. XK201114) and Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions. We are grateful to the technical support from National Key Clinical Department of Laboratory Medicine of Jiangsu Province Hospital.
Supplementary Table 1—Target sites and sequences of shRNA plasmids.
Compliance with ethical standards
Conflict of interest
The authors declare no conflicts of interest.
All procedures performed in studies involving human participants were in accordance with ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.
- Jemal A, Siegel R, Xu J, Ward E (2010) Cancer statistics, 2010. CA: Cancer J. Clinic 60:277–300Google Scholar