Biotechnology Letters

, Volume 39, Issue 5, pp 701–710 | Cite as

MiRNA-145 suppresses lung adenocarcinoma cell invasion and migration by targeting N-cadherin

  • Dongping Mo
  • Daheng Yang
  • Xuelian Xiao
  • Ruihong Sun
  • Lei Huang
  • Jian Xu
Original Research Paper



To investigate the roles of miR-145 in lung adenocarcinoma (LAC) and to clarify the regulation of N-cadherin by miR-145.


In 57 paired clinical LAC tissues, diminished miR-145 was significantly correlated with the lymph node metastasis and was negatively correlated with N-cadherin mRNA level expression. Wound healing and transwell assays revealed a reduced capability of tumor metastasis induced by miR-145 in LAC. miR-145 negatively regulated the invasion of cell lines through targeting N-cadherin by directly binding to its 3′-untranslated region. Silencing of N-cadherin inhibited invasion and migration of LAC cell lines similar to miR-145 overexpression.


MiR-145 could inhibit invasion and migration of lung adenocarcinoma cell lines by directly targeting N-cadherin.


N-Cadherin Invasion Lung adenocarcinoma Migration miR-145 Non-small cell lung cancer 



This study was supported by Key Laboratory for Laboratory Medicine of Jiangsu Province of China (No. XK201114) and Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions. We are grateful to the technical support from National Key Clinical Department of Laboratory Medicine of Jiangsu Province Hospital.

Supporting information

Supplementary Table 1—Target sites and sequences of shRNA plasmids.

Compliance with ethical standards

Conflict of interest

The authors declare no conflicts of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Supplementary material

10529_2017_2290_MOESM1_ESM.doc (30 kb)
Supplementary material 1 (DOC 30 kb)


  1. Ashaie MA, Chowdhury EH (2016) Cadherins: the superfamily critically involved in breast cancer. Curr Pharm Des 22:616–638CrossRefPubMedGoogle Scholar
  2. Bartel DP (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116:281–297CrossRefPubMedGoogle Scholar
  3. Calin GA, Croce CM (2006) MicroRNA signatures in human cancers. Nat Rev Cancer 6:857–866CrossRefPubMedGoogle Scholar
  4. Chen CZ (2005) MicroRNAs as oncogenes and tumor suppressors. N Engl J Med 353:1768–1771CrossRefPubMedGoogle Scholar
  5. Gao P et al (2013) The molecular mechanism of microRNA-145 to suppress invasion-metastasis cascade in gastric cancer. Oncogene 32:491–501CrossRefPubMedGoogle Scholar
  6. Hazan RB, Qiao R, Keren R, Badano I, Suyama K (2004) Cadherin switch in tumor progression. Ann NY Acad Sci 1014:155–163CrossRefPubMedGoogle Scholar
  7. Hulit J et al (2007) N-Cadherin signaling potentiates mammary tumor metastasis via enhanced extracellular signal-regulated kinase activation. Cancer Res 67:3106–3116CrossRefPubMedGoogle Scholar
  8. Jemal A, Siegel R, Xu J, Ward E (2010) Cancer statistics, 2010. CA: Cancer J. Clinic 60:277–300Google Scholar
  9. Lee JM, Dedhar S, Kalluri R, Thompson EW (2006) The epithelial-mesenchymal transition: new insights in signaling, development, and disease. J Cell Biol 172:973–981CrossRefPubMedPubMedCentralGoogle Scholar
  10. Liu X et al (2009) Uncovering growth-suppressive MicroRNAs in lung cancer. Clin Canc Res 15:1177–1183CrossRefGoogle Scholar
  11. Qin X, Yan L, Zhao X, Li C, Fu Y (2012) microRNA-21 overexpression contributes to cell proliferation by targeting PTEN in endometrioid endometrial cancer. Oncol Lett 4:1290–1296PubMedPubMedCentralGoogle Scholar
  12. Takai Y, Ikeda W, Ogita H, Rikitake Y (2008) The immunoglobulin-like cell adhesion molecule nectin and its associated protein afadin. Annu Rev Cell Develop Biol 24:309–342CrossRefGoogle Scholar
  13. Tanaka H et al (2010) Monoclonal antibody targeting of N-cadherin inhibits prostate cancer growth, metastasis and castration resistance. Nat Med 16:1414–1420CrossRefPubMedPubMedCentralGoogle Scholar
  14. Vora N, Reckamp KL (2008) Non-small cell lung cancer in the elderly: defining treatment options. Semin Oncol 35:590–596CrossRefPubMedPubMedCentralGoogle Scholar
  15. Yin R et al (2011) microRNA-145 suppresses lung adenocarcinoma-initiating cell proliferation by targeting OCT4. Oncol Rep 25:1747–1754PubMedGoogle Scholar
  16. Zhang J et al (2013a) MiR-124 suppresses growth of human colorectal cancer by inhibiting STAT3. PLoS ONE 8:e70300CrossRefPubMedPubMedCentralGoogle Scholar
  17. Zhang X et al (2013b) N-Cadherin expression is associated with acquisition of EMT phenotype and with enhanced invasion in erlotinib-resistant lung cancer cell lines. PLoS ONE 8:e57692CrossRefPubMedPubMedCentralGoogle Scholar
  18. Zheng M, Wu Z, Wu A, Huang Z, He N, Xie X (2016) MiR-145 promotes TNF-alpha-induced apoptosis by facilitating the formation of RIP1-FADDcaspase-8 complex in triple-negative breast cancer. Tumour 37:8599–8607CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Dongping Mo
    • 1
  • Daheng Yang
    • 1
  • Xuelian Xiao
    • 1
  • Ruihong Sun
    • 1
  • Lei Huang
    • 1
  • Jian Xu
    • 1
    • 2
  1. 1.Department of Laboratory MedicineThe First Affiliated Hospital of Nanjing Medical UniversityNanjingPeople’s Republic of China
  2. 2.National Key Clinical Department of Laboratory MedicineNanjingChina

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