Abstract
BmK CT, one of the key toxins in the venom of the scorpion, Buthus martensii Karsch, can interact specifically with glioma cells as a chloride channel blocker and inhibit the invasion and migration of those cells via MMP-2. A recombinant adenovirus, Ad-BmK CT, was constructed and characterized by in vitro and in vivo studies, using MTT cytotoxicity assay and the glioma C6/RFP (red fluorescence protein)/BALB/c allogeneic athymic nude mice model, respectively. The adenovirus-mediated expression of BmK CT displayed a high activity in suppressing rat C6 glioma cells growth and invasion thereby suggesting that this recombinant adenovirus may be a powerful method for treating glioblastoma.
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Acknowledgments
This project is supported by grants from “National Natural Science Foundation of China (No. 31272100, 31071924)”, the “National High Technology Research and Development Program of China (863 Program, No. 2012AA020809)”, and “the Program for the Top Young Academic Leaders of Higher Learning Institutions of Shanxi”. We wish to thank Prof. Hong Tang (Institute of Biophysics, Chinese Academy of Sciences) for technical advice on the animal experiments. We will also like to thank Shuhua Zheng (University of Miami Miller School of Medicine) for his comments and grammar revision of the paper.
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Du, J., Fu, Y., Wang, J. et al. Adenovirus-mediated expression of BmK CT suppresses growth and invasion of rat C6 glioma cells. Biotechnol Lett 35, 861–870 (2013). https://doi.org/10.1007/s10529-013-1167-9
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DOI: https://doi.org/10.1007/s10529-013-1167-9