Abstract
Violacein and deoxyviolacein display a broad range of interesting biological properties but their production is rarely distinguished due to the lack of suitable analytical methods. An HPLC method has been developed for the separation and quantification of violacein and deoxyviolacein and can determine the content of both molecules in microbial cultures. A comparison of different production microorganisms, including recombinant Escherichia coli and the natural producer Janthinobacterium lividum, revealed that the formation of violacein and deoxyviolacein is strain-specific but showed significant variation during growth although the ratio between the two compounds remained constant.
Similar content being viewed by others
References
Andrighetti-Fröhner CR, Antonio RV, Creczynski-Pasa TB, Barardi CRM, Simões CMO (2003) Cytotoxicity and potential antiviral evaluation of violacein produced by Chromobacterium violaceum. Mem Inst Oswaldo Cruz 98:843–848
Bromberg N, Dreyfuss JL, Regatieri CV, Palladino MV, Duran N, Nader HB, Haun M, Justo GZ (2010) Growth inhibition and pro-apoptotic activity of violacein in Ehrlich ascites tumor. Chem Biol Interact 186(1):43–52
Jiang P, Wang H, Zhang C, Lou K, Xing XH (2009) Reconstruction of the violacein biosynthetic pathway from Duganella sp. B2 in different heterologous hosts. Appl Microbiol Biotechnol 86(4):1077–1088
May G, Brummer B, Ott H (1991) Treatment of prophylaxis of polio and herpes virus infections–comprises admin. of 3-(1,2-dihydro-5-(5-hydroxy-1H- indol-3-yl)-2-oxo-3Hpyrrole-3-ylidene)-1,3-dihydro-2H-indol-2-one. DE 3935066 Patent
Mendes AS, de Carvalho JE, Duarte MCT, Duran N, Bruns RE (2001) Factorial design and response surface optimization of crude violacein for Chromobacterium violaceum production. Biotechnol Lett 23(23):1963–1969
Pantanella F, Berlutti F, Passariello C, Sarli S, Morea C, Schippa S (2007) Violacein and biofilm production in Janthinobacterium lividum. J Appl Microbiol 102(4):992–999
Rettori D, Durán N (1998) Production, extraction and purification of violacein: an antibiotic pigment produced by Chromobacterium violaceum. World J Microbiol Biotechnol 14(5):685–688
Rodrigues AL (2007) Heterologous production of violacein in Escherichia coli. Master Dissertation, Federal University of Santa Catarina, Florianópolis
Sarovich DS, Pemberton JM (2007) pPSX: a novel vector for the cloning and heterologous expression of antitumor antibiotic gene clusters. Plasmid 57(3):306–313
Wang H, Jiang P, Lu Y, Ruan Z, Jiang R, Xing XH, Lou K, Wei D (2009) Optimization of culture conditions for violacein production by a new strain of Duganella sp. B2. Biochem Eng J 44(2–3):119–124
Wille G, Steglich W (2001) A short synthesis of the bacterial pigments violacein and deoxyviolacein. Synthesis 5:759–762
Acknowledgments
We acknowledge Prof. John Pemberton for the kind donation of the plasmids pJP1000 and pPSX-Vio+. André L. Rodrigues gratefully acknowledges financial support by the German Academic Exchange Service (A-08-71658) and by the Max-Buchner Foundation (Nr. 2924).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Rodrigues, A.L., Göcke, Y., Bolten, C. et al. Microbial production of the drugs violacein and deoxyviolacein: analytical development and strain comparison. Biotechnol Lett 34, 717–720 (2012). https://doi.org/10.1007/s10529-011-0827-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-011-0827-x