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Raw starch fermentation to ethanol by an industrial distiller’s yeast strain of Saccharomyces cerevisiae expressing glucoamylase and α-amylase genes

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Abstract

Industrial strains of a polyploid, distiller’s Saccharomyces cerevisiae that produces glucoamylase and α-amylase was used for the direct fermentation of raw starch to ethanol. Strains contained either Aspergillus awamori glucoamylase gene (GA1), Debaryomyces occidentalis glucoamylase gene (GAM1) or D. occidentalis α-amylase gene (AMY), singly or in combination, integrated into their chromosomes. The strain expressing both GA1 and AMY generated 10.3% (v/v) ethanol (80.9 g l−1) from 20% (w/v) raw corn starch after 6 days of fermentation, and decreased the raw starch content to 21% of the initial concentration.

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Acknowledgments

This work was supported by a grant of the Korea Ministry of Education, Science and Technology (The Regional Core Research Program/Biohousing Research Institute). Ha-Ram Kim and Young-Kum Im were supported by the second stage of the Brain Korea 21 project.

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Authors and Affiliations

  1. Department of Biological Sciences, College of Natural Sciences, Chonnam National University, Gwangju, 500-757, South Korea

    Ha-Ram Kim, Young-Kum Im, Il-Chul Kim, Hwanghee Blaise Lee & Suk Bai

  2. Department of Microbiology, College of Medicine, Seonam University, Namwon, Chunpook, South Korea

    Hyun-Mi Ko

  3. Department of Cosmetology, Dongkang College University, Gwangju, South Korea

    Jong-Eon Chin

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  1. Ha-Ram Kim
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  2. Young-Kum Im
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  3. Hyun-Mi Ko
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  4. Jong-Eon Chin
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  5. Il-Chul Kim
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  6. Hwanghee Blaise Lee
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  7. Suk Bai
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Correspondence to Suk Bai.

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Kim, HR., Im, YK., Ko, HM. et al. Raw starch fermentation to ethanol by an industrial distiller’s yeast strain of Saccharomyces cerevisiae expressing glucoamylase and α-amylase genes. Biotechnol Lett 33, 1643–1648 (2011). https://doi.org/10.1007/s10529-011-0613-9

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  • DOI: https://doi.org/10.1007/s10529-011-0613-9

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