Abstract
To improve the secretion and expression of human interferon alpha 2b (IFN) in Lactococcus lactis, a synthetic pro-peptide, LEISSTCDA (LEISS), was fused to the N-terminus of IFN. This gave a higher secretion efficiency (12% vs. 5%) and yield (~2.8-fold) of IFN. The signal peptide, SPSlpA (SlpA, an S-layer protein of Lactobacillus brevis), was also tested to secrete IFN instead of SPUsp45 (Usp45, the main secreted protein in L. lactis). This gave increased IFN secretion (~3-fold) but lower total production. All the recombinant IFN had appropriate bioactivities in an antiviral assay.
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Acknowledgments
This work was supported by grants from National Program for High Technology Research and Development of China (2006AA10Z319) and the Key Project of the Chinese Academy of Sciences (KSCXZ-YW-G-016). We greatly appreciate Ms. Ling Fu and Dr. Xiaoling Liu for their kindly help for analysis of the biological activity of IFN.
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Zhang, Q., Zhong, J., Liang, X. et al. Improvement of human interferon alpha secretion by Lactococcus lactis . Biotechnol Lett 32, 1271–1277 (2010). https://doi.org/10.1007/s10529-010-0285-x
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DOI: https://doi.org/10.1007/s10529-010-0285-x