Abstract
cDNAs encoding for five mAChR subtypes (M1–M5) were cloned under different promoters in various eukaryotic vectors and each subtype was expressed in different mammalian cell lines. CHO-K1 cell line was the best for generating stable cell lines expressing muscarinic receptors. Immunofluorescence and flow cytometry revealed that expression of M1–M5 was primarily localized on the cell membrane. Western blotting and radio-ligand binding studies revealed that expression of each receptor was stable at higher passages.
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Received 22 September 2005; Revisions requested 5 October 2005; Revisions received 2 November 2005; Accepted 4 November 2005
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Khattar, S.K., Bora, R.S., Priyadarsiny, P. et al. High Level Stable Expression of Pharmacologically Active Human M1–M5 Muscarinic Receptor Subtypes in Mammalian Cells. Biotechnol Lett 28, 121–129 (2006). https://doi.org/10.1007/s10529-005-5130-2
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DOI: https://doi.org/10.1007/s10529-005-5130-2