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Effects of Some Growth Factors and Cytokines on the Expression of the Repair Enzyme MGMT and Protein MARP in Human Cells In Vitro

Effect of Some Growth Factors and Cytokines

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Abstract

The inducible repair enzyme O6-methylguanine-DNA methyltransferase (MGMT) eliminates O6-methylguanine adducts in DNA and protects the cells from damaging effects of alkylating agents. We have found that anti-MGMT antibodies recognize both the MGMT protein with a mol. weight ~ 24 kDa and a protein with a mol. weight ~ 48 kDa, which was named MARP (anti-methyltransferase antibody recognizable protein). A number of growth factors and cytokines were shown to regulate the expression of MGMT and MARP proteins. The ranges of concentrations of several growth factors and cytokines that caused increasing or decreasing protein amounts in human cell cultures were determined. The results of special biological experiments have allowed us to assume a possible role of MARP in the repair of alkyl adducts in human cells.

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Acknowledgements

We are very grateful to Professors Korneluyk A.I., Kuchuk M.V., Drs. Chernykh S.I., Potopalsky A.I., and Luchakovskaya Yu.S. for providing the preparations of cytokines and alkylating agent.

Funding

The study was supported by the National Academy of Sciences of Ukraine (No. 0115U000355) and the Czech Research Infrastructure for Systems Biology C4SYS (Project No. LM2015055).

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Authors and Affiliations

  1. University of South Bohemia in České Budějovice, Branisovska 1760, 37005, Ceske Budejovice, Czech Republic

    Kateryna Kotsarenko

  2. Institute of Molecular Biology and Genetics of NAS of Ukraine, Kiev, 03680, Ukraine

    Valentyna Lylo, Tetiana Ruban, Larysa Macewicz & Lyubov Lukash

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  1. Kateryna Kotsarenko
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  2. Valentyna Lylo
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  3. Tetiana Ruban
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  4. Larysa Macewicz
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  5. Lyubov Lukash
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Correspondence to Kateryna Kotsarenko.

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Kotsarenko, K., Lylo, V., Ruban, T. et al. Effects of Some Growth Factors and Cytokines on the Expression of the Repair Enzyme MGMT and Protein MARP in Human Cells In Vitro. Biochem Genet 56, 459–477 (2018). https://doi.org/10.1007/s10528-018-9854-9

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