Abstract
To detect DNA polymorphisms in the peanut, we screened 26 polymorphic primers using intron–exon splice junction (ISJ), universal rice primer (URP), and directed amplification of minisatellite region DNA (DAMD) techniques. Amplification of genomic DNA of 16 peanut accessions yielded 121 ISJ, 50 URP, and 25 DAMD fragments, of which 34, 25 and 16 were polymorphic, respectively. The range of polymorphism was 10.0–62.5%, averaging 27.7%, for ISJ; 20–80%, averaging 49.5%, for URP; and 28.6–50.0%, averaging 36.3%, for DAMD. In comparisons of multiplex ratio, average polymorphism information content, and marker index, the URP markers were relatively more efficient than ISJ and DAMD markers. Clustering results remained more or less the same with ISJ and URP markers. To the best of our knowledge, this is the first report on the study of the genetic diversity of the peanut using ISJ, URP, and DAMD markers.
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Acknowledgments
This research was supported by grants from the National Natural Science Foundation of China (31240059, 31160294), earmarked fund for Modern Agro-industry Technology Research System (2009BADA8B03), Guangxi Science and Technology Project (GKG1222009-2C, GKG10100004-7), Guangxi Science Fund (2011GXNSFA018079, 2012GXNSFBA053051), Guangxi Key Laboratory Construction Project (12-071-09), Guangxi Academy of Agricultural Sciences Fund (GNK2013JQ05, GNK2013JQ03, GNK2011YZ09, GNK2012YM22, GNK2012YZ03, GNK2012JM15), and Open Project Program of State Key Laboratory of Food Science and Technology, Nanchang University (SKLF-KF-201209).
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Faqian Xiong and Junxian Liu have contributed equally to this study.
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Xiong, F., Liu, J., Jiang, J. et al. Molecular Profiling of Genetic Variability in Domesticated Groundnut (Arachis hypogaea L.) Based on ISJ, URP, and DAMD Markers. Biochem Genet 51, 889–900 (2013). https://doi.org/10.1007/s10528-013-9615-8
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DOI: https://doi.org/10.1007/s10528-013-9615-8