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Biological and Molecular Characterization of a Crucifer Tobamovirus Infecting Oilseed Rape

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Abstract

In China, the tobamovirus that infects oilseed rape has been misdiagnosed as Tobacco mosaic virus (TMV) based on its morphological similarity and serological relatedness. Recently, a tobamovirus has been isolated from oilseed rape in China, which we named Youcai mosaic virus Br (YoMV-Br), according to its biological and molecular characteristics. It had strong infectivity to Cruciferae but less to Solanaceae, Leguminosae, and Cucurbitaceae, and its virion morphology was consistent with that of the tobamoviruses. At high concentrations, it serologically cross reacted with TMV antiserum. The 3′ terminal sequence (2,283 nucleotides) of YoMV-Br was determined, including the 3′ noncoding region, the CP and MP genes, and the C-terminal part of the replicase gene. Between the MP and CP genes, 77 nucleotides overlapped. Compared with homologous regions of 21 recognized species of Tobamovirus, YoMV-Br had a much higher identity to crucifer species than to other tobamoviruses. Phylogenetic analysis demonstrated that YoMV-Br was closely related to the YoMV cluster of tobamoviruses and distantly to TMV, so that they likely belong to different strains of the same species.

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Authors and Affiliations

  1. Hubei Crop Disease Monitoring and Safety Control Key Laboratory, Department of Plant Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China

    Li Cai & Mingsheng Hou

  2. Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, 430062, Wuhan, China

    Li Cai, Kunrong Chen, Xuejiang Zhang, Liying Yan & Zeyong Xu

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  1. Li Cai
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  2. Kunrong Chen
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  3. Xuejiang Zhang
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  4. Liying Yan
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  5. Mingsheng Hou
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  6. Zeyong Xu
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Correspondence to Li Cai or Zeyong Xu.

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Cai, L., Chen, K., Zhang, X. et al. Biological and Molecular Characterization of a Crucifer Tobamovirus Infecting Oilseed Rape. Biochem Genet 47, 451–461 (2009). https://doi.org/10.1007/s10528-009-9244-4

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  • DOI: https://doi.org/10.1007/s10528-009-9244-4

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