Abstract
Onion leaf blight, caused by Botrytis squamosa (Walker), is a destructive disease of onion. Conidia produced on overwintered sclerotia are the main source of initial inoculum, and those produced on lesions are responsible for secondary inoculum build-up. The biological control agent Microsphaeropsis ochracea (Carisse & Bernier) was evaluated for its ability to control sclerotia-borne inoculum, to colonize onion leaves and reduce the production of conidia under field conditions. Colonisation by M. ochracea of onion leaves at different growth stages was monitored and its effect on B. squamosa sporulation on necrotic leaves was evaluated. Onion plots were treated with either Dithane® or with M. ochracea at 7–10-day intervals and according to inoculum production index (IPI). The concentration of airborne conidia and the number of lesions per leaf, on 20 plants per plot, were evaluated throughout the cropping season. The number of conidia produced per sclerotium treated with M. ochracea, was reduced by 75.5%. In the field, M. ochracea colonised only senescent or necrotic leaves and reduced the production of conidia on these leaves by an average of 82% as compared with untreated leaves. Best disease control was obtained by Dithane®, followed by M. ochracea applied at 7–10-day intervals. For the three years of the study, there were no significant differences in airborne concentrations of conidia in plots treated at 7–10-day intervals with Dithane® or M. ochracea. Fall application of M. ochracea could be used as a sanitation practice to reduce initial inoculum or as a part of an IPM program during the season.
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References
S.C. Alderman M.L. Lacy (1983) ArticleTitleInfluence of dew period and temperature on infection of onion leaves by dry conidia of Botrytis squamosa Phytopathology 73 1020–1023
Balis, F.J. and J.W. Lorbeer, 1992. The physiology of conidiation in Botrytis squamosa. In: K. Verhoeff, N.E. Malathrakis and B. Williamson (eds), Recent Advances in Botrytis Research. Pudoc Scientific Publishers, Wageningen, The Netherlands. Proceedings of the 10th International Botrytis Symposium. Heraklion, Crete, Greece. April 5–10, 1992, pp. 123–126.
M. Benyagoub N. Benhamou O. Carisse (1998) ArticleTitleCytochemical investigation of the antagonistic interaction between Microsphaeropsis sp. (isolate P130A) and Venturia inaequalis Phytopathology 88 605–613
R.R. Bergquist J.W. Lorbeer (1972) ArticleTitleApothecial production, compatibility and sex in Botryotinia squamosa Mycologia 64 1270–1281
J. Bernier O. Carisse T.C. Paulitz (1996) ArticleTitleFungal communities isolated from dead apple leaves from orchards in Quebec Phytoprotection 77 129–134
I. Bujold T.C. Paulitz O. Carisse (2001) ArticleTitleEffect of Microsphaeropsis sp. on the production perithecia and ascospsores of Gibberella zeae Plant Dis. 85 977–984
O. Carisse J. Bernier (2002a) ArticleTitleComparison of four Microsphaeropsis isolates: biological control potential and effect of environmental factors on growth, pycnidial production and spore germination Mycol. Res. 106 1455–1462 Occurrence Handle10.1017/S0953756202006858
O. Carisse S. El Bassam N. Benhamou (2001) ArticleTitleEffect of Microsphaeropsis sp. (strain P130A) on germination and production of sclerotia of Rhizoctonia solani and interaction between the antagonist and the pathogen Phytopathology 91 782–791
O. Carisse J. Bernier (2002b) ArticleTitleMicrosphaeropsis ochracea sp. nov. associated with dead apple leaves Mycologia 94 297–301
O. Carisse V. Philion D. Rolland J. Bernier (2000) ArticleTitleEffect of fall application of fungal antagonists on spring ascospore production of apple scab pathogen, Venturia inaequalis Phytopathology 90 31–37 Occurrence Handle1:CAS:528:DC%2BD3cXlt1ylsw%3D%3D
C.A. Clark (1976) Histopathology and leaf surface relationships of B otrytis squamosa and B. cinerea on onion Cornell University Ithaca, NY 134
C.A. Clark J.W. Lorbeer (1976) ArticleTitleComparative histopathology of Botrytis squamosa and B. cinerea on onion leaves Phytopathology 66 1279–1289
C.A. Clark J.W. Lorbeer (1977) ArticleTitleThe role of phyllosphere bacteria in pathogenesis by B. squamosa and B. cinerea on onion leaves Phytopathology 67 96–100 Occurrence Handle1:CAS:528:DyaE2sXhtFOkt74%3D
L. Currah R.B. Maude (1984) ArticleTitleLaboratory tests for leaf resistance to Botrytis squamosa in onions Ann. Appl. Biol. 105 277–283
S. El Bassam N. Benhamou O. Carisse (2002) ArticleTitleThe role of melanin in the antagonistic interaction between the apple scab pathogen Venturia inaequalis and Microsphaeropsis ochracea Can. J. Microbiol. 48 349–358 Occurrence Handle1:CAS:528:DC%2BD38XjvVGgsLs%3D Occurrence Handle12030708
L.A. Ellerbrock J.W. Lorbeer (1977a) ArticleTitleSources of primary inoculum of Botrytis squamosa Phytopathology 67 363–372
L.A. Ellerbrock J.W. Lorbeer (1977b) ArticleTitleSurvival of sclerotia and conidia and of Botrytis squamosa Phytopathology 67 219–225
N.J. Fokkema J.W. Lorbeer (1974) ArticleTitleInteractions between Alternaria porri and saprophytic mycoflora of onion leaves Phytopathology 64 1128–1133
Jarvis, W.R., 1977. Botryotinia and Botrtis species: Taxonomy, physiology and pathogenicity: a guide to the literature. Monograph No.15. Eds Agriculture Canada. 195 pp.
J. Köhl W.M.L. Molhoek C.H. Plas Particlevan der W.M.L. Molhoek G.J.T. Kessel N.J. Fokkema (1992) Biological control of Botrytis leaf blight of onion: significance of sporulation suppression K. Verhoeff N.E. Malathrakis B. Williamson (Eds) Recent Advances in Botrytis Research Pudoc Scientific Publishers Wageningen 192–196
J. Köhl C.H. Plas Particlevan der W.M.L. Molhoek N.J. Fokkema (1995) ArticleTitleEffect of interrupted leaf wetness periods on suppression of sporulation of Botrytis alii and B. cinerea by antagonists on dead onion leaves Eur. J. Plant Pathol. 101 627–637
M.L. Lacy G.A. Pontius (1983) ArticleTitlePrediction of Weather-Mediated release of conidia of Botrytis squamosa from onion leaves in the fields Phytopathology 73 670–676
J.W. Lorbeer (1992) Botrytis leaf blight of onion H.S. Chaube U.S. Singh A.N. Mukhopadhyay J. Kumar (Eds) Plant Disease of International Importance Prentice Hall Englewood Cliffs New Jersey 186–211
J.W. Lorbeer L.A. Ellerbrock (1976) ArticleTitleFailure of Ethylene Bisdithiocarbamates to control botrytis leaf blight of onion Proc. Am. Phytopathol. Soc. 3 75–84 Occurrence Handle1:CAS:528:DyaE2sXhvVCnsr0%3D
J.W. Lorbeer P.C. Vincelli (1990) ArticleTitleEfficacy of dicarboximides fungicides and fungicide combinations for control of Botrytis leaf blight of onion in New York Plant Dis. 74 235–237 Occurrence Handle1:CAS:528:DyaK3cXkslaqsLc%3D
M.P. McQuilken S.P. Budge J.M. Whipps (1997) ArticleTitleBiological control of Sclerotia sclerotiorum by film-coating Conithyrium minitans on to sunflower seed and sclerotia Plant Pathol. 46 919–929 Occurrence Handle10.1046/j.1365-3059.1997.d01-79.x
E.F. Schneider K.J. Dickert (1994) ArticleTitleHealth costs and benefits of fungicides used in Agriculture: a literature review J. Agromed. 1 19–37
R.G.D. Steel J.H. Torrie (1980) Principles and Procedures of Statistics, A Biometrical Approach EditionNumber2 McGraw-Hill Book Company New York 633
J.C. Sutton T.D.W. James P.M. Rowell (1983) ArticleTitleRelation of weather and host factors to an epidemic of botrytis leaf blight in onions Can. J. Plant Pathol. 5 256–265
J.C. Sutton C.J. Swanton T.J. Gillespie (1978) ArticleTitleRelation of weather variables and host factors to incidence of airborne spores of Botrytis squamosa Can. J. Bot. 56 2460–2469
G.J. Turner H.T. Tribe (1976) ArticleTitleOn Coniothyrium minitans and its parasitism of Sclerotinia species T. Brit. Mycol. Soc. 66 97–105
D.M. Tremblay B.G. Talbot O. Carisse (2003) ArticleTitleSensitivity to different classes of fungicides in Botrytis squamosa Plant Dis. 87 573–578 Occurrence Handle1:CAS:528:DC%2BD3sXjslSlu7o%3D
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Carisse, O., Rolland, D. & Tremblay, DM. Effect of Microsphaeropsis ochracea on Production of Sclerotia-borne and Airborne Conidia of Botrytis squamosa. Biocontrol 51, 107–126 (2006). https://doi.org/10.1007/s10526-005-5807-4
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DOI: https://doi.org/10.1007/s10526-005-5807-4