Spatial Organization of the Transport of Interstitial Fluid and Lymph in Rat Liver (Scanning Electron Microscopy of Injection Replicas)

Using the method of scanning electron microscopy of injection replicas, we studied the movement of a new injection mass between the blood microcirculation system, interstitial space, lymphatic system, and bile transport system in rat liver under normal conditions and 3 days after the occlusion of the common bile duct. The casts of the perisinusoidal spaces of Disse’s after injection of the injection mass through the portal vein and common bile duct were obtained. Their direct transition not only in “leakages” structurally related to lymphatic capillaries in interlobular spaces, but also in perivascular spaces around the portal and hepatic veins. The flow of the injection mass through the perivascular spaces leads to the formation of peculiar “sheaths” around hepatic veins and components of the portal complex. The proposed approach allows effective visualization of the structural basis of interaction of various compartments of the fluid microcirculation in the liver under normal and pathological conditions.

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  1. 1.

    Debie P, Hernot S. Emerging Fluorescent Molecular Tracers to Guide Intra-Operative Surgical Decision-Making. Front. Pharmacol. 2019;10:510.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  2. 2.

    Dongaonkar RM, Stewart RH, Quick CM, Uray KL, Cox CS. Jr, Laine GA. Hepatic transudation barrier properties. Microcirculation. 2018;25(2).

  3. 3.

    Jessen NA, Munk AS, Lundgaard I, Nedergaard M. The Glymphatic System: A Beginner’s Guide. Neurochem Res. 2015;40(12):2583-2599.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  4. 4.

    Lukacs-Kornek V. The Role of Lymphatic Endothelial Cells in Liver Injury and Tumor Development. Front. Immunol. 2016;7:548.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  5. 5.

    McCuskey RS. The hepatic microvascular system in health and its response to toxicants. Anat Rec (Hoboken). 2008; 291(6):661-671.

    Article  PubMed  Google Scholar 

  6. 6.

    Ohtani O, Ohtani Y. Hepatic Lymphatic System. Encyclopedia of Medical Immunology. Mackay IR, Rose NR, Diamond B, Davidson A, eds. Springer, 2014.

  7. 7.

    Ohtani O, Ohtani Y. Lymph circulation in the liver. Anat. Rec. (Hoboken). 2008;291(6):643-652.

    Article  PubMed  Google Scholar 

  8. 8.

    Rasmussen MK, Mestre H, Nedergaard M. The glymphatic pathway in neurological disorders. Lancet Neurol. 2018; 17(11):1016-1024.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  9. 9.

    Scanning Electron Microscopy of Vascular Casts: Methods and Applications. Motta P, Murakami T, Fujita H, eds. Electron Microscopy in Biology and Medicine. Springer, 2012. Vol. 10.

  10. 10.

    Stücker O, Pons-Himbert C, Laemmel E. Towards a better understanding of lymph circulation. Phlebolymphology. 2008;15(1):31-35.

    Google Scholar 

  11. 11.

    Sulaberidze GD, Kardzvia DDzh, Kikalishvili LA, Khomeriki TsT. Common intralobular microcirculatory module peculliarities in cholestasis in white rats. Georgian Med. News. 2006;(132):100-105.

  12. 12.

    Tanaka M, Iwakiri Y. Lymphatics in the liver. Curr. Opin. Immunol.2018;53:137-142.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  13. 13.

    Tsomaia K, Patarashvili L, Bebiashvili I, Azmaiparashvili E, Kakabadze M, Jalabadze N, Sareli M, Gusev S, Kordzaia D. New corrosion cast media and its ability for SEM and light microscope investigation. Microsc. Res. Tech. 2020;83(7):778-789.

    CAS  Article  PubMed  Google Scholar 

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Correspondence to S. A. Gusev.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 170, No. 9, pp. 395-400, September, 2020

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Patarshvili, L.G., Tsomaia, K.B., Bebiashvili, I.S. et al. Spatial Organization of the Transport of Interstitial Fluid and Lymph in Rat Liver (Scanning Electron Microscopy of Injection Replicas). Bull Exp Biol Med 170, 395–399 (2021).

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Key Words

  • liver
  • injection replicas
  • lymphatic circulation
  • interstitial fluid