Bulletin of Experimental Biology and Medicine

, Volume 164, Issue 6, pp 780–783 | Cite as

Expression of Bcl-2 Family Proteins in the Ovarian Follicular Apparatus in the Acute Period after Experimental Hyperthermia

  • S. V. Michurina
  • S. I. Kolesnikov
  • A. L. Bochkareva
  • S. A. Arkhipov
  • I. Yu. Ishchenko

The expression of apoptosis regulators (proapoptotic protein Bad and anti-apoptotic protein Bcl-2) was analyzed and Bcl-2/Bad ratio in the follicular apparatus of the rat ovary was determined on day 3 after hyperthermia (rectal temperature 43.5°C). Hyperthermia in the catabolic phase leads to different degrees of activation of the molecular “switches” of apoptosis in cells of ovarian follicular epithelium. This was seen from increased intensity of immunohistochemical staining for Bad protein against the background of more pronounced expression of Bcl-2 protein. On day 3 after exposure to hyperthermia, Bcl-2/Bad ratio increased, which reflects antiapoptotic protection of cells and conditions for blockade of mitochondrial pathway of apoptosis in the follicular apparatus of the ovaries during the acute period after hyperthermia.

Key Words

apoptosis hyperthermia ovarian follicular apparatus Bcl-2/Bad 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • S. V. Michurina
    • 1
  • S. I. Kolesnikov
    • 2
  • A. L. Bochkareva
    • 1
  • S. A. Arkhipov
    • 1
  • I. Yu. Ishchenko
    • 1
  1. 1.Research Institute of Clinical and Experimental Lymphology, Affiliated Branch of the Federal Research Centre Institute of Cytology and GeneticsSiberian Division of the Russian Academy of ScienceNovosibirskRussia
  2. 2.Research Center for Family Health and Human Reproduction ProblemsIrkutskRussia

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