Advertisement

Bulletin of Experimental Biology and Medicine

, Volume 164, Issue 6, pp 775–779 | Cite as

Induced Pluripotent Stem Cell-Derived Hematopoietic Embryoid Bodies Secrete Sphingosine-1-Phosphate and Revert Endothelial Injury

  • S. Kasuda
  • R. Kudo
  • K. Yuui
  • Y. Sakurai
  • K. Hatake
EXPERIMENTAL METHODS FOR CLINICAL PRACTICE

The possibility of sphingosine-1-phosphate production by induced pluripotent stem cells is examined to assess their potential in treatment of sepsis. The hematopoietic embryoid bodies were derived from the culture of 6-day-old differentiated induced pluripotent stem cells. These embryoid bodies secreted sphingosine-1-phosphate, an important bioactive lipid that regulates integrity of the pulmonary endothelial barrier, prevents elevation of its permeability, and impedes the formation of stress fibers in human endotheliocytes derived from umbilical vein. The data attest to potentiality of induced pluripotent stem cells in treatment of sepsis.

Key Words

endotheliocytes induced pluripotent stem cells sepsis sphingosine-1-phosphate 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Cribbs SK, Matthay MA, Martin GS. Stem cells in sepsis and acute lung injury. Crit. Care Med. 2010;38(12):2379-2385.CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Garcia JG, Liu F, Verin AD, Birukova A, Dechert MA, Gerthoffer WT, Bamberg JR, English D. Sphingosine 1-phosphate promotes endothelial cell barrier integrity by Edg-dependent cytoskeletal rearrangement. J. Clin. Invest. 2001;108(5):689-701.CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Kasuda S, Tatsumi K, Sakurai Y, Kato J, Taminishi S, Takeda T, Ohashi K, Okano T, Hatake K, Shima M. Expression of coagulation factors from murine induced pluripotent stem cell-derived liver cells. Blood Coagul. Fibrinolysis. 2011; 22(4):271-279.CrossRefPubMedGoogle Scholar
  4. 4.
    Komarova YA, Mehta D, Malik AB. Dual regulation of endothelial junctional permeability. Sci. STKE. 2007;2007(412):re8.CrossRefPubMedGoogle Scholar
  5. 5.
    Mao M, Wang SN, Lv XJ, Wang Y, Xu JC. Intravenous delivery of bone marrow-derived endothelial progenitor cells improves survival and attenuates lipopolysaccharide-induced lung injury in rats. Shock. 2010;34(2):196-204.CrossRefPubMedGoogle Scholar
  6. 6.
    Mei SH, Haitsma JJ, Dos Santos CC, Deng Y, Lai PF, Slutsky AS, Liles WC, Stewart DJ. Mesenchymal stem cells reduce inflammation while enhancing bacterial clearance and improving survival in sepsis. Am. J. Respir. Crit. Care Med. 2010;182(8):1047-1057.CrossRefPubMedGoogle Scholar
  7. 7.
    Pappu R, Schwab SR, Cornelissen I, Pereira JP, Regard JB, Xu Y, Camerer E, Zheng YW, Huang Y, Cyster JG, Coughlin S.R. Promotion of lymphocyte egress into blood and lymph by distinct sources of sphingosine-1-phosphate. Science. 2007; 316:295-298.CrossRefPubMedGoogle Scholar
  8. 8.
    Sammani S, Moreno-Vinasco L, Mirzapoiazova T, Singleton PA, Chiang ET, Evenoski CL, Wang T, Mathew B, Husain A, Moitra J, Sun X, Nunez L, Jacobson JR, Dudek SM, Natarajan V, Garcia JG. Differential effects of sphingosine-1-phosphate receptors on airway and vascular barrier function in the murine lung. Am. J. Respir. Cell Mol. Biol. 2010;43(4):394-402.CrossRefPubMedGoogle Scholar
  9. 9.
    Siehler S, Manning DR. Pathways of transduction engaged by sphingosine 1-phosphate through G protein-coupled receptors. Biochim. Biophys. Acta. 2002;1582(1-3):94-99.CrossRefPubMedGoogle Scholar
  10. 10.
    Cribbs SK, Martin GS. Stem cells in sepsis and acute lung injury. Am. J. Med. Sci. 2011;341(4):325-332.CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Venkataraman K, Lee YM, Michaud J, Thangada S, Ai Y, Bonkovsky HL, Parikh NS, Habrukowich C, Hla T. Vascular endothelium as a contributor of plasma sphingosine 1-phosphate. Circ. Res. 2008;102(6):669-676.CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Wolfson RK, Chiang ET, Garcia JG. HMGB1 induces human lung endothelial cell cytoskeletal rearrangement and barrier disruption. Microvasc. Res. 2011;81(2):189-197.CrossRefPubMedGoogle Scholar
  13. 13.
    Wu KH, Wu HP, Chan CK, Hwang SM, Peng CT, Chao YH. The role of mesenchymal stem cells in hematopoietic stem cell transplantation: from bench to bedsides. Cell Transplant. 2013;22(4):723-729.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • S. Kasuda
    • 1
  • R. Kudo
    • 1
  • K. Yuui
    • 1
  • Y. Sakurai
    • 2
  • K. Hatake
    • 1
  1. 1.Department of Legal MedicineNara Medical UniversityKashiharaJapan
  2. 2.Department of PediatricsMatsubara Tokushukai HospitalMatsubaraJapan

Personalised recommendations