Effect of Nanocrystalline Particles of Chitin on Blood Components in Humans and Experimental Animals

Nanocrystalline particles of chitin in the form of hydrosol in a concentration of 0.63 mg/ml have no effect on aggregation of human platelets and clotting time of platelet-poor plasma in coagulation tests. ADP-induced aggregation of human platelets was inhibited by these nanoparticles in concentrations of 0.63 and 1.00 mg/ml in comparison with the control. Intravenous administration of nanoparticles in a dose of 1 mg/kg to guinea pigs produced no anticoagulant effect. The nanocrystalline particles of chitin can be of interest as potential drug delivery systems.

This is a preview of subscription content, access via your institution.

References

  1. 1.

    Bender EA, Adorne MD, Colomé LM, Abdalla DS, Guterres SS, Pohlmann AR. Hemocompatibility of poly(ε-caprolactone) lipid-core nanocapsules stabilized with polysorbate 80-lecithin and uncoated or coated with chitosan. Int. J. Pharm. 2012;426(1-2):271-279.

    CAS  Article  PubMed  Google Scholar 

  2. 2.

    Born GV. Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature. 1962;194:927-929.

    CAS  Article  PubMed  Google Scholar 

  3. 3.

    Cardénas G, Cabrera G, Taboada E, Miranda SP. Chitin characterization by SEM, FTIR, XRD, and 13C cross polarization/ mass angle spinning NMR. J. Appl. Pol. Sci. 2004;93(4):1876-1885. doi: https://doi.org/10.1002/app.20647.

    Article  Google Scholar 

  4. 4.

    Chen C, Li D, Yano H, Abe K. Dissolution and gelation of α-chitin nanofibers using a simple NaOH treatment at low temperatures. Cellulose. 2014;21(5):3339-3346.

    CAS  Article  Google Scholar 

  5. 5.

    Cheng J, Feng S, Han S, Zhang X, Chen Y, Zhou X, Wang R, Li X, Hu H, Zhang J. Facile assembly of cost-effective and locally applicable or injectable nanohemostats for hemorrhage control. ACS Nano. 2016;10(11):9957-9973.

    CAS  Article  PubMed  Google Scholar 

  6. 6.

    Ding F, Deng H, Du Y, Shi X, Wang Q. Emerging chitin and chitosan nanofibrous materials for biomedical applications. Nanoscale. 2014;6(16):9477-9493.

    CAS  Article  PubMed  Google Scholar 

  7. 7.

    Fornaguera C, Solans C. Methods for the in vitro characterization of nanomedicines-biological component interaction. J. Pers Med. 2017;7(1). pii: E2. doi: https://doi.org/10.3390/jpm7010002.

  8. 8.

    Ilinskaya AN, Dobrovolskaia MA. Nanoparticles and the blood coagulation system. Part II: safety concerns. Nanomedicine (Lond). 2013;8(6):969-981.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  9. 9.

    Islam N, Ferro V. Recent advances in chitosan-based nanoparticulate pulmonary drug delivery. Nanoscale. 2016;8(30):14 341-14 358.

    CAS  Article  Google Scholar 

  10. 10.

    Kim ES, Lee JS, Lee HG. Nanoencapsulation of red ginseng extracts using chitosan with polyglutamic acid or fucoidan for improving antithrombotic activities. J. Agric. Food Chem. 2016;64(23):4765-4771.

    CAS  Article  PubMed  Google Scholar 

  11. 11.

    Pereira AG, Muniz EC, Hsieh YL. Chitosan-sheath and chitin-core nanowhiskers. Carbohydr. polym. 2014;107:158-166.

    CAS  Article  PubMed  Google Scholar 

  12. 12.

    Simon TL, Hyers TM, Gaston JP, Harker LA. Heparin pharmacokinetics increased requirements in pulmonary embolism. Br. J. Haematol. 1978;39(1):111-120.

    CAS  Article  PubMed  Google Scholar 

  13. 13.

    Sithole MN, Choonara YE, du Toit LC, Kumar P, Pillay V. A review of semi-synthetic biopolymer complexes: modified polysaccharide nano-carriers for enhancement of oral drug bioavailability. Pharm. Dev. Technol. 2017;22(2):283-295.

    CAS  Article  PubMed  Google Scholar 

  14. 14.

    Song L, Zhu D, Liu L, Dong X, Zhang H, Leng X. Evaluation of the coagulation properties of arginine-chitosan/DNA nanoparticles. J. Biomed. Mater. Res. B Appl. Biomater. 2010;95(2):374-379.

    Article  PubMed  Google Scholar 

  15. 15.

    Zeng JB, He YS, Li SL, Wang YZ. Chitin whiskers: an overview. Biomacromolecules. 2012;13(1):1-11.

    CAS  Article  PubMed  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to N. N. Drozd.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 164, No. 12, pp. 739-743, December, 2017

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Drozd, N.N., Torlopov, M.A., Udoratina, E.V. et al. Effect of Nanocrystalline Particles of Chitin on Blood Components in Humans and Experimental Animals. Bull Exp Biol Med 164, 766–769 (2018). https://doi.org/10.1007/s10517-018-4076-z

Download citation

Key Words

  • chitin
  • nanoparticles
  • plasma/blood clotting