Modeling of 3D Structure of Chimeric Constructs Based on Hemagglutinin of Influenza Virus and Immunogenic Epitopes of Streptococcus Agalactiae

  • E. A. Fedorova
  • T. A. Smolonogina
  • I. N. Isakova-Sivak
  • D. A. Koren’kov
  • T. S. Kotomina
  • G. F. Leont’eva
  • A. N. Suvorov
  • L. G. Rudenko
VIROLOGY
  • 2 Downloads

A project of an experimental recombinant vector vaccine for prevention of diseases caused by pathogenic streptococci based on ScaAB lipoprotein of Streptococcus agalactiae and a coldadapted strain of live influenza vaccine as a vector was developed. The sequence of ScaAB lipoprotein was analyzed and fragments forming immunodominant epitopes were determined. Chimeric molecules of influenza virus hemagglutinin H7 carrying insertions of bacterial origin were constructed. Based on the results of simulation, the most promising variants were selected; they represented fragments of lipoprotein ScaAB lacking N-terminal domain bound to hemagglutinin via a flexible linker. These insertions should minimally modulate the properties of the influenza strain, while retaining potential immunogenicity to a wide group of pathogenic streptococci.

Key Words

vector vaccine influenza viruses group B Streptococci 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • E. A. Fedorova
    • 1
  • T. A. Smolonogina
    • 1
  • I. N. Isakova-Sivak
    • 1
  • D. A. Koren’kov
    • 1
  • T. S. Kotomina
    • 1
  • G. F. Leont’eva
    • 2
  • A. N. Suvorov
    • 2
  • L. G. Rudenko
    • 1
  1. 1.A. A. Smorodintsev Department of VirologyInstitute of Experimental MedicineSt. PetersburgRussia
  2. 2.Department of Molecular MicrobiologyInstitute of Experimental MedicineSt. PetersburgRussia

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