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In Vitro Model for Studying of the Role of IGFBP6 Gene in Breast Cancer Metastasizing

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Bulletin of Experimental Biology and Medicine Aims and scope

IGFBP6 gene plays an important role in the pathogenesis of breast cancer. In this work, we performed knockdown of IGFBP6 gene in MDA-MB-231 cells and obtained a stable cell line. Knockdown of IGFBP6 gene was confirmed by the real-time PCR. The influence of IGFBP6 gene on migration and proliferation of breast cancer cells was studied. Knockdown of IGFBP6 gene reduced migration activity of MDA-MB-231 cells and increased their proliferation rate. This in vitro cell model can be used for the further analysis of the role of IGFBP6 gene in the pathogenesis of breast cancer.

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Authors and Affiliations

  1. BioClinicum Center, Moscow, Russia

    S. V. Nikulin, M. P. Raigorodskaya, A. A. Poloznikov & G. S. Zakharova

  2. Moscow Institute of Physics and Technology (MIPT), Moscow, Russia

    S. V. Nikulin

  3. University Medical Center Hamburg-Eppendorf, Hamburg, Germany

    U. Schumacher, D. Wicklein, C. Stürken & K. Riecken

  4. P. A. Hertsen Moscow Oncology Research Center, Branch of Branch of National Medical Research Radiology Center of the Ministry of Health of the Russian Federation, Moscow, Russia

    K. A. Fomicheva, B. Ya. Alekseev & M. Yu. Shkurnikov

Authors
  1. S. V. Nikulin
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  2. M. P. Raigorodskaya
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  3. A. A. Poloznikov
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  4. G. S. Zakharova
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  5. U. Schumacher
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  6. D. Wicklein
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  7. C. Stürken
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  8. K. Riecken
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  9. K. A. Fomicheva
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  10. B. Ya. Alekseev
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  11. M. Yu. Shkurnikov
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Corresponding author

Correspondence to M. Yu. Shkurnikov.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 164, No. 11, pp. 646-651, November, 2017

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Nikulin, S.V., Raigorodskaya, M.P., Poloznikov, A.A. et al. In Vitro Model for Studying of the Role of IGFBP6 Gene in Breast Cancer Metastasizing. Bull Exp Biol Med 164, 688–692 (2018). https://doi.org/10.1007/s10517-018-4060-7

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  • DOI: https://doi.org/10.1007/s10517-018-4060-7

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