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Bulletin of Experimental Biology and Medicine

, Volume 164, Issue 5, pp 624–628 | Cite as

Study of the Oxime-induced Reactivation of Acetyl- and Butyrylcholinesterase of Human with Inhibition of Organophosphorus Insecticide In Vitro

  • A. M. Kolesnikov
  • M. A. Yuidin
  • A. S. Nikiforov
  • I. M. Ivanov
  • N. G. Vengerovich
  • A. S. Makacheev
Article

The efficiency of different reactivators of cholinesterase (toxogonin, dipiroxime, pralidoxime, carboxim, HI-6, and methoxime) at inhibition of butyrylcholinesterase and human acetylcholinesterase by organophosphate insecticide malathion was evaluated in in vitro experiments. Most reactivators increased inhibition of butyrylcholinesterase in comparison with the control, but HI-6 in a concentration of 10—3 mol/liter partially (10%) restored activity of the enzyme. Oxime-induced reactivation of acetylcholinesterase was most pronounced in dipyroxime and toxogonin: parameters of the kinetics of reduction of the phosphorylated enzyme differed by more than 2 times from the values received with the use of other reactivators.

Key Words

malathion inhibition of cholinesterase cholinesterase reactivators reactivation of acetyl- and butyrylcholinesterase 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • A. M. Kolesnikov
    • 1
  • M. A. Yuidin
    • 1
  • A. S. Nikiforov
    • 1
  • I. M. Ivanov
    • 1
  • N. G. Vengerovich
    • 1
  • A. S. Makacheev
    • 1
  1. 1.State Research Testing Institute of Military MedicineMinistry of Defense of the Russian FederationSt. PetersburgRussia

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