Bulletin of Experimental Biology and Medicine

, Volume 164, Issue 4, pp 543–549 | Cite as

In Vitro Modeling of Co-Transplantation of Multipotent Stromal Mesenchymal Cells from Orbital Fat Pad and Lipoaspirate of Human Subcutaneous Adipose Tissue in Organ Culture in Collagen Gel

  • S. A. Borzenok
  • D. S. Afanas’eva
  • M. B. Gushchina
  • D. S. Ostrovskii
  • S. P. Domogatsky
  • E. O. Osidak
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)

The interplay of multipotent stromal cells derived from the orbital fat pads and cells of the lipoaspirate from the subcutaneous adipose tissue was studied using in vitro co-transplantation model in an organ culture in a collagen gel. Microscopy findings and intensity of apoptosis and cell proliferation in cultures of lipoaspirate with and without multipotent stromal cells showed that the cells maintained their viability, proliferation capacity, and cytokine secretion activity. Higher proliferatitive activity of cells in cocultures promotes renewal of fat transplant cells and can help to maintain its stable volume in delayed terms after transplantation.

Key Words

organ culture apoptosis multipotent stromal cells lipofilling enophthalmos 


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  1. 1.
    Borzenok SA, Gushchina MB, Afanasyeva DS, Shilkin GA. Orbital adipose tissue is a new resource for transplantology. Vestn. Transplantol. Iskusstv. Organov. 2015;17(4):118-123. Russian.Google Scholar
  2. 2.
    Malakhovskaya VI, Visaitova ZU. Eight years of experience in practical clinical lipofilling application: results estimation. Vestn. Estet. Med. 2010;9(3):64-74. Russian.Google Scholar
  3. 3.
    Borzenok SA, Gushchina MB, Afanaseva DS. Patent RU No. 2609657. Method for extracting mesenchymal stem cells from orbital adipose tissue. Bull. No. 4. Published October 22, 2015.Google Scholar
  4. 4.
    Ryzhevsky DV, Pashtaev NP, Pozdeyeva NA, Trubin VV. The use of lipofilling in the treatment of post-traumatic enophthalmos. Prakt. Med. 2016;(2-1):43-45. Russian.Google Scholar
  5. 5.
    Freshney RI. Culture of Animal Cells: A Manual of Basic Technique. Moscow, 2010. Russian.Google Scholar
  6. 6.
    Bauer SM, Bauer RJ, Liu ZJ, Chen H, Goldstein L, Velazquez OC. Vascular endothelial growth factor-C promotes vasculogenesis, angiogenesis, and collagen constriction in three-dimensional collagen gels. J. Vasc. Surg. 2005;41(4):699-707.CrossRefPubMedGoogle Scholar
  7. 7.
    Boschert MT, Beckert BW, Puckett CL, Concannon MJ. Analysis of lipocyte viability after liposuction. Plast. Reconstr. Surg. 2002;109(2):761-765.CrossRefPubMedGoogle Scholar
  8. 8.
    Brown M, Lee M, Zwiebel S, Adenuga P, Molavi S, Gargesha M, Varghai D, Guyuron B. Augmentation of intraorbital volume with fat injection. Plast. Reconstr. Surg. 2014;133(5):1098-1106.PubMedGoogle Scholar
  9. 9.
    Cakir B, Aygit AC, Omur-Okten O, Yalcin O. Retro-orbital intraconal fat injection: an experimental study in rabbits. J. Oral Maxillofac. Surg. 2012;70(1):242-250.CrossRefPubMedGoogle Scholar
  10. 10.
    Cortese A, Savastano G, Felicetta L. Free fat transplantation for facial tissue augmentation. J. Oral Maxillofac. Surg. 2000;58(2):164-169.CrossRefPubMedGoogle Scholar
  11. 11.
    Carswell KA, Lee M, Fried SK. Culture of isolated human adipocytes and isolated adipose tissue. Methods Mol. Biol. 2012;806:203-214.CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Hardy TG, Joshi N, Kelly MH. Orbital volume Augmentation with autologous micro-fat grafts. Ophthal. Plastic Reconstr. Surg. 2007;23(6):445-449.CrossRefGoogle Scholar
  13. 13.
    Horwitz EM, Le Blanc K, Dominici M, Mueller I, Slaper-Cortenbach I, Marini FC, Deans RJ, Krause DS, Keating A; International Society for Cellular Therapy. Clarification of the nomenclature for MSC: The international society for cellular therapy position statement. Cytotherapy. 2005;7(5):393-395.Google Scholar
  14. 14.
    International Guiding Principles for Biomedical Research Involving Animals. The Development of Science-based Guidelines for Laboratory Animal Care: Proceedings of the November 2003 International Workshop / National Research Council (US) Institute for Laboratory Animal Research. Washington, 2004. URL:
  15. 15.
    Lee JY, Lee KH, Shin HM, Chung KH, Kim GI, Lew H. Orbital volume augmentation after injection of human orbital adipose-derived stem cells in rabbits. Invest. Ophthalmol. Vis. Sci. 2013;54(4):2410-2416.CrossRefPubMedGoogle Scholar
  16. 16.
    Matsumoto D, Sato K, Gonda K, Takaki Y, Shigeura T, Sato T, Aiba-Kojima E, Iizuka F, Inoue K, Suga H, Yoshimura K. Cell-assisted lipotransfer: Supportive use of human adiposederived cells for soft tissue augmentation with lipoinjection. Tissue Eng. 2006;12(12):3375-3382.CrossRefPubMedGoogle Scholar
  17. 17.
    Nae S, Bordeianu I, Stăncioiu A.T, Antohi N. Human adiposederived stem cells: definition, isolation, tissue-engineering applications. Rom. J. Morphol. Embryol. 2013;54(4):919-924.Google Scholar
  18. 18.
    Oedayrajsingh-Varma MJ, van Ham SM, Knippenberg M, Helder MN, Klein-Nulend J, Schouten TE, Ritt MJ, van Milligen FJ. Adipose tissue-derived mesenchymal stem cell yield and growth characteristics are affected by the tissue-harvesting procedure. Cytotherapy. 2006;8(2):166-177.CrossRefPubMedGoogle Scholar
  19. 19.
    Sonoda E, Aoki S, Uchihashi K, Soejima H, Kanaji S, Izuhara K, Satoh S, Fujitani N, Sugihara H, Toda S. A new organotypic culture of adipose tissue fragments maintains viable mature adipocytes for a long term, together with development of immature adipocytes and mesenchymal stem cell-like cells. Endocrinology. 2008;149(10):4794-4798.CrossRefPubMedGoogle Scholar
  20. 20.
    Tchkonia T, Giorgadze N, Pirtskhalava T, Tchoukalova Y, Karagiannides I, Forse RA, DePonte M, Stevenson M, Guo W, Han J, Waloga G, Lash TL, Jensen MD, Kirkland JL. Fat depot origin affects adipogenesis in primary cultured and cloned human preadipocytes. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2002;282(5):R1286-R1296.CrossRefPubMedGoogle Scholar
  21. 21.
    Yoshimura K, Suga H, Eto H. Adipose-derived stem/progenitor cells: roles in adipose tissue remodeling and potential use for soft tissue augmentation. Regen. Med. 2009;4(2):265-273.CrossRefPubMedGoogle Scholar
  22. 22.
    Yoshimura K, Sato K, Aoi N, Kurita M, Inoue K, Suga H, Eto H, Kato H, Hirohi T, Harii K. Cell-assisted lipotransfer for facial lipoatrophy: efficacy of clinical use of adipose-derived stem cells. Dermatol. Surg. 2008;34(9):1178-1185.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • S. A. Borzenok
    • 1
    • 2
  • D. S. Afanas’eva
    • 1
  • M. B. Gushchina
    • 3
  • D. S. Ostrovskii
    • 1
    • 4
  • S. P. Domogatsky
    • 5
    • 6
  • E. O. Osidak
    • 6
    • 7
  1. 1.S. N. Fyodorov Eye Microsurgery Federal State InstitutionMinistry of Health of the Russian FederationMoscowRussia
  2. 2.A. I. Evdokimov Moscow State University of Medicine and DentistryMinistry of Health of the Russian FederationMoscowRussia
  3. 3.Central Research Institute of Dentistry and Maxillofacial SurgeryMinistry of Health of the Russian FederationMoscowRussia
  4. 4.Research Institute of General Pathology and PathophysiologyMinistry of Health of the Russian FederationMoscowRussia
  5. 5.National Research Medical Center of CardiologyMinistry of Health of the Russian FederationMoscowRussia
  6. 6.Imtek CompanyMoscowRussia
  7. 7.N. F. Gamaleya Federal Research Center of Epidemiology and MicrobiologyMinistry of Health of the Russian FederationMoscowRussia

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