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Bulletin of Experimental Biology and Medicine

, Volume 164, Issue 4, pp 543–549 | Cite as

In Vitro Modeling of Co-Transplantation of Multipotent Stromal Mesenchymal Cells from Orbital Fat Pad and Lipoaspirate of Human Subcutaneous Adipose Tissue in Organ Culture in Collagen Gel

  • S. A. Borzenok
  • D. S. Afanas’eva
  • M. B. Gushchina
  • D. S. Ostrovskii
  • S. P. Domogatsky
  • E. O. Osidak
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)

The interplay of multipotent stromal cells derived from the orbital fat pads and cells of the lipoaspirate from the subcutaneous adipose tissue was studied using in vitro co-transplantation model in an organ culture in a collagen gel. Microscopy findings and intensity of apoptosis and cell proliferation in cultures of lipoaspirate with and without multipotent stromal cells showed that the cells maintained their viability, proliferation capacity, and cytokine secretion activity. Higher proliferatitive activity of cells in cocultures promotes renewal of fat transplant cells and can help to maintain its stable volume in delayed terms after transplantation.

Key Words

organ culture apoptosis multipotent stromal cells lipofilling enophthalmos 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • S. A. Borzenok
    • 1
    • 2
  • D. S. Afanas’eva
    • 1
  • M. B. Gushchina
    • 3
  • D. S. Ostrovskii
    • 1
    • 4
  • S. P. Domogatsky
    • 5
    • 6
  • E. O. Osidak
    • 6
    • 7
  1. 1.S. N. Fyodorov Eye Microsurgery Federal State InstitutionMinistry of Health of the Russian FederationMoscowRussia
  2. 2.A. I. Evdokimov Moscow State University of Medicine and DentistryMinistry of Health of the Russian FederationMoscowRussia
  3. 3.Central Research Institute of Dentistry and Maxillofacial SurgeryMinistry of Health of the Russian FederationMoscowRussia
  4. 4.Research Institute of General Pathology and PathophysiologyMinistry of Health of the Russian FederationMoscowRussia
  5. 5.National Research Medical Center of CardiologyMinistry of Health of the Russian FederationMoscowRussia
  6. 6.Imtek CompanyMoscowRussia
  7. 7.N. F. Gamaleya Federal Research Center of Epidemiology and MicrobiologyMinistry of Health of the Russian FederationMoscowRussia

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