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Role of Matrix Metalloproteinase-2 in the Development of Cyclophosphamide-Induced Cardiomyopathy

  • MORPHOLOGY AND PATHOMORPHOLOGY
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Bulletin of Experimental Biology and Medicine Aims and scope

Immunohistochemical assay was employed to determine localization of MMP-2 in cardiomyocytes of WAG rats and changes in MMP-2 expression during modeled cardiomyopathy induced by single intraperitoneal injection of cyclophosphamide (125 mg/kg) alone or in combination with preventive intraperitoneal administration of an equal dose of asparcam-L (potassium-magnesium asparaginate) 30 min prior to the cytostatic. In the myocardium of control and experimental rats, MMP-2 was mostly located in cardiomyocyte nuclei. During the development of cyclophosphamide-induced cardiomyopathy (in 3 days after injection), the index of MMP-2-positive cardiomyocyte nuclei increased by 76%. In contrast to control hearts, MMP-2 was also expressed in the cardiomyocyte sarcoplasm. Preventive injection of asparcam-L moderated the cardiotoxic effect of cyclophosphamide, which manifested in less pronounced increase in the volume density of cardiomyocytes with lytic changes (by 42%) and index of MMP-2+ cardiomyocyte nuclei (by 23%) in comparison with the rats exposed to cyclophosphamide alone.

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Authors and Affiliations

  1. Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia

    E. V. Koldysheva, M. G. Klinnikova, D. B. Nikityuk, E. K. Ivleva, N. A. Listvyagova & E. L. Lushnikova

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  1. E. V. Koldysheva
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  2. M. G. Klinnikova
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  3. D. B. Nikityuk
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  4. E. K. Ivleva
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  5. N. A. Listvyagova
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  6. E. L. Lushnikova
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Corresponding author

Correspondence to E. V. Koldysheva.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 164, No. 10, pp. 484-489, October, 2017

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Koldysheva, E.V., Klinnikova, M.G., Nikityuk, D.B. et al. Role of Matrix Metalloproteinase-2 in the Development of Cyclophosphamide-Induced Cardiomyopathy. Bull Exp Biol Med 164, 483–487 (2018). https://doi.org/10.1007/s10517-018-4017-x

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  • DOI: https://doi.org/10.1007/s10517-018-4017-x

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