Bulletin of Experimental Biology and Medicine

, Volume 164, Issue 4, pp 442–445 | Cite as

α-Tocopherol Reduces Morphological Changes and Oxidative Stress during Gentamicin-Induced Acute Renal Failure

  • N. Stojiljkovic
  • S. Ilic
  • M. Veljkovic
  • J. Todorovic
  • M. Mladenovic

We studied the effect of α-tocopherol on gentamicin-induced morphological and functional changes in the kidneys of Wistar rats. Special attention was paid to the ability of α-tocopherol administered in combination with gentamicin to correct ultrastructural changes in the glomerular basal membrane and tubules. Combined treatment with α-tocopherol (100 mg/kg) and gentamicin (100 mg/kg) led to correction of histopathological and biochemical changes and oxidative injury to the kidneys induced by this antibiotic.

Key Words

α-tocopherol gentamicin glomerular basal membrane Wistar rats 


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  1. 1.
    Abdel-Naim AB, Abdel-Wahab MH, Attia FF. Protective effects of vitamin E and probucol against gentamicin-induced nephrotoxicity in rats. Pharmacol. Res. 1999;40(2):183-187.CrossRefPubMedGoogle Scholar
  2. 2.
    Abd Hamid NA, Hasrul MA, Ruzanna RJ, Ibrahim IA, Baruah PS, Mazlan M, Yusof YA, Ngah WZ. Effect of vitamin E (Tri E®) on antioxidant enzymes and DNA damage in rats following eight weeks exercise. J. Nutr. 2011;10:37. doi: Scholar
  3. 3.
    Balakumar P, Rohilla A, Thangathirupathi A. Gentamicininduced nephrotoxicity: Do we have a promising therapeutic approach to blunt it? Pharmacol. Res. 2010;62(3):179-186.CrossRefPubMedGoogle Scholar
  4. 4.
    Forrest VJ, Kang YH, McClain DE, Robinson DH, Ramakrishnan N. Oxidative stress-induced apoptosis prevented by Trolox. Free Radic. Biol. Med. 1994;16(6):675-684.CrossRefPubMedGoogle Scholar
  5. 5.
    Martínez-Salgado C, Eleno N, Morales AI, Pérez-Barriocanal F, Arévalo M, López-Novoa JM. Gentamicin treatment induces simultaneous mesangial proliferation and apoptosis in rats. Kidney Int. 2004;65(6):2161-2171.CrossRefPubMedGoogle Scholar
  6. 6.
    Martínez-Salgado C, López-Hernández FJ, López-Novoa JM. Glomerular nephrotoxicity of aminoglycosides. Toxicol. Appl. Pharmacol. 2007;223(1):86-98.CrossRefPubMedGoogle Scholar
  7. 7.
    Morales AI, Detaille D, Prieto M, Puente A, Briones E, Arévalo M, Leverve X, López-Novoa JM, El-Mir MY. Metformin prevents experimental gentamicin-induced nephropathy by a mitochondria-dependent pathway. Kidney Int. 2010;77(10):861-869.CrossRefPubMedGoogle Scholar
  8. 8.
    Nakazawa T, Miyanoki Y, Urano Y, Uehara M, Saito Y, Noguchi N. Effect of vitamin E on 24(S)-hydroxycholesterolinduced necroptosis-like cell death and apoptosis. J. Steroid Biochem. Mol. Biol. 2016. Mar 4. pii: S0960-0760(16)30049-8. doi: 10.1016/j.jsbmb.2016.03.003.Google Scholar
  9. 9.
    Negrette-Guzmán M, García-Niño WR, Tapia E, Zazueta C, Huerta-Yepez S, León-Contreras JC, Hernández-Pando R, Aparicio-Trejo OE, Madero M, Pedraza-Chaverri J. Curcumin Attenuates Gentamicin-Induced Kidney Mitochondrial Alterations: Possible Role of a Mitochondrial Biogenesis Mechanism. Evid. Based. Complement. Alternat. Med. 2015;2015. ID 917435. doi: 10.1155/2015/917435.Google Scholar
  10. 10.
    Stojiljkovic N, Ilic S, Veljkovic M, Todorovic J. Effects of supplementation with vitamin e on gentamycin-induced acute renal failure in rats. Facta Universitatis. Ser. Med. Biol. 2014;16(2):61-66.Google Scholar
  11. 11.
    Stojiljkovic N, Mihailovic D, Veljkovic S, Stoiljkovic M, Jovanovic I. Glomerular basement membrane alterations induced by gentamicin administration in rats. Exp. Toxicol. Pathol. 2008;60(1):69-75.CrossRefPubMedGoogle Scholar
  12. 12.
    Quiros Y, Vicente-Vicente L, Morales AI, López-Novoa JM, López-Hernández FJ. An integrative overview on the mechanisms underlying the renal tubular cytotoxicity of gentamicin. Toxicol. Sci. 2011;119(2):245-256.CrossRefPubMedGoogle Scholar
  13. 13.
    Yoshikawa Y, Hizume K, Oda Y, Takeyasu K, Araki S, Yoshikawa K. Protective effect of vitamin C against doublestrand breaks in reconstituted chromatin visualized by singlemolecule observation. Biophys. J. 2006;90(3):993-999.CrossRefPubMedGoogle Scholar
  14. 14.
    Zakharova IO, Sokolova TV, Avrova NF. Α-tocopherol prevents ERK1/2 activation in PC12 cells under conditions of oxidative stress and its contribution to the protective effect. Bull. Exp. Biol. Med. 2013;155(1):44-47.Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • N. Stojiljkovic
    • 1
  • S. Ilic
    • 1
  • M. Veljkovic
    • 1
  • J. Todorovic
    • 2
  • M. Mladenovic
    • 2
  1. 1.Institute of PhysiologyNisSerbia
  2. 2.Faculty of MedicineUniversity of NisNisSerbia

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