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Bulletin of Experimental Biology and Medicine

, Volume 164, Issue 3, pp 402–403 | Cite as

A Technique for In Vitro Studying of the Permeability of the Spinal Cord Dura Mater

  • V. V. Krasnov
  • M. V. Stogov
  • T. A. Silant’eva
  • N. V. Kubrak
  • E. A. Kireeva
METHODS
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We propose an in vitro method for studying permeability of spinal cord dura mater for components of autological serum using an original device. Sixty native samples of the spinal cord dura mater obtained from 12 mongrel dogs were used for testing of the device. The coefficient of permeability variation (V) for blood serum substances did not exceed 5% in most cases excluding lactate (V=8.03%). Analysis of spinal cord dura mater permeability in vitro for various substances using the developed device provides reproducible results with acceptable variability (5-10%).

Key Words

diffusion chamber permeability dura mater of spinal cord 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • V. V. Krasnov
    • 1
  • M. V. Stogov
    • 1
  • T. A. Silant’eva
    • 1
  • N. V. Kubrak
    • 1
  • E. A. Kireeva
    • 1
  1. 1.Russian Ilizarov Scientific Center for Restorative Traumatology and Orthopaedics, Ministry of Health of the Russian FederationKurganRussia

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