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Bulletin of Experimental Biology and Medicine

, Volume 164, Issue 3, pp 397–401 | Cite as

Dynamics of Distribution of Capillaries with Matrix Metalloproteinase-2 and Its Tissue Inhibitor in Rat Brain during Development of Experimental Hypertension

  • V. M. Chertok
  • A. G. Chertok
  • N. V. Zakharchuk
  • V. A. Nevzorova
Article
  • 24 Downloads

The capillaries containing MMP-2 and its tissue inhibitor TIMP-2 were examined in cerebral cortex and white matter obtained from intact Wistar rats (n=5) and the rats with progressing experimental renovascular hypertension (n=35). In hypertensive rats, the changes in intensity of the immunohistochemical reaction and in the density of capillaries expressing TIMP-2 significantly differed from the corresponding values in MMP-2-positive capillaries, which resulted in pronounced deviation of MMP-2/TIMP-2 index from the control level (especially in cerebral cortex) probably attesting to enhanced risk of complications in cases with arterial hypertension.

Key Words

cerebral capillaries immunolocalization of MMP-2 and TIMP-2 renovascular hypertension 

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References

  1. 1.
    Miromanova NA, Miromanov AM. Changes in the system “matrix metalloprotease — inhibitor of matrix metalloprotease” at children’s influenza. Fundament. Issled. 2014(10-2):328-332. Russian.Google Scholar
  2. 2.
    Motavkin PA, Chertok VM, Shul’ga SD. Effect of acetylcholine on mast cells of the dura mater. Bull. Exp. Biol. Med. 1979;87(5):518-521.CrossRefGoogle Scholar
  3. 3.
    Poteriaeva ON. Matrix metalproteinases: structure, regulation, role in pathological states development (literature review). Meditsina Obrazovanie Sibiri. 2010(5):7-17. Russian.Google Scholar
  4. 4.
    Chertok VM. Mast cells of the outer sheath of cerebral basilar arteries. Morfologiya. 1980;79(11):72-79. Russian.Google Scholar
  5. 5.
    Chertok VM, Startseva МS, Kotsyuba AYe. The application of a “pixel method” for the quantitative assessment of the results of the histochemical studies. Morfologiya. 2012;142(5):71-75. Russian.Google Scholar
  6. 6.
    Chertok VM, Chertok AG. Regulatory capacity of the brain capillaries. Tikhookean. Med. Zh. 2016(2):72-80. Russian.Google Scholar
  7. 7.
    Candelario-Jalil E, Yang Y, Rosenberg GA. Diverse roles of matrix metalloproteinases and tissue inhibitors of metalloproteinases in neuroinflammation and cerebral ischemia. Neuroscience. 2009;158(3):983-994.CrossRefPubMedGoogle Scholar
  8. 8.
    Chertok VM, Kotsyuba AE. The Neurochemical features of intranuclear neurons of the medulla oblongata in normo- and hypertensive rats. Neurochem. J. 2016;10(3):232-239.CrossRefGoogle Scholar
  9. 9.
    Cunningham LA, Wetzel M, Rosenberg GA. Multiple roles for MMPs and TIMPs in cerebral ischemia. Glia. 2005;50(4):329-339.CrossRefPubMedGoogle Scholar
  10. 10.
    Florczak-Rzepka M, Grond-Ginsbach C, Montaner J, Steiner T. Matrix metalloproteinases in human spontaneous intracerebral hemorrhage: an update. Cerebrovasc. Dis. 2012;34(4):249-262.CrossRefPubMedGoogle Scholar
  11. 11.
    Grandas OH, Mountain DH, Kirkpatrick SS, Cassada DC, Stevens SL, Freeman MB, Goldman MH. Regulation of vascular smooth muscle cell expression and function of matrix metalloproteinases is mediated by estrogen and progesterone exposure. J. Vasc. Surg. 2009;49(1):185-191.CrossRefPubMedGoogle Scholar
  12. 12.
    Hiller O, Lichte A, Oberpichler A, Kocourek A, Tschesche H. Matrix metalloproteinases collagenase-2, macrophage elastase, collagenase-3, and membrane type 1-matrix metalloproteinase impair clotting by degradation of fibrinogen and factor XII. J. Biol. Chem. 2000;275(42):33 008-33 013.CrossRefGoogle Scholar
  13. 13.
    Johnson C, Galis ZS. Matrix metalloproteinase-2 and -9 differentially regulate smooth muscle cell migration and cellmediated collagen organization. Arterioscler. Thromb. Vasc. Biol. 2004;24(1):54-60.CrossRefPubMedGoogle Scholar
  14. 14.
    Lakhan SE, Kirchgessner A, Tepper D, Leonard A. Matrix metalloproteinases and blood-brain barrier disruption in acute ischemic stroke. Front. Neurol. 2013;4:32. doi:  https://doi.org/10.3389/fneur.2013.00032.PubMedPubMedCentralGoogle Scholar
  15. 15.
    Rempe RG, Hartz AM, Bauer B. Matrix metalloproteinases in the brain and blood-brain barrier: Versatile breakers and makers. J. Cereb. Blood Flow Metab. 2016;36(9):1481-1507.CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • V. M. Chertok
    • 1
  • A. G. Chertok
    • 1
  • N. V. Zakharchuk
    • 2
  • V. A. Nevzorova
    • 2
  1. 1.Department of Human AnatomyVladivostokRussia
  2. 2.Institute of Therapy and Instrumental Diagnostics, Pacific State Medical University, Ministry of Health of the Russian FederationVladivostokRussia

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