Inactivation of M111 Protein Gene Modifies Streptococcus Pyogenes Interactions with Mouse Macrophages In Vitro
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Immunomodulatory properties of S. pyogenes protein M111 were studied on the model of Gurov strain and its isogenic mutant not expressing M protein. Mouse resident peritoneal macrophages were incubated with bacteria and generation of nitroxide and superoxide anions and production of IL-6, IL-10, and IL-17 were evaluated. Protein M111 modified macrophage response: it exhibited antiphagocytic activity, prevented ROS formation, and stimulated the production of anti-inflammatory cytokine IL-10. The results suggested that this protein could serve in the bacteria as a factor suppressing the host defense forces and promoting the realization of the strategy beneficial for pathogens — escape from the host immune defense.
Key WordsStreptococcus pyogenes M protein macrophages superoxide radicals cytokines
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- 2.Suvorova MA, Tsapieva AN, Duplik NV, Kramskaya TA, Grabovskaya KB, Kiseleva EP, Chereshnev VA, Suvorov AN. Construction of a Streptococcus strain mutant by M protein gene. Med. Akad. Zh. 2016;16(4):235-236. Russian.Google Scholar
- 6.Dileepan T, Linehan JL, Moon JJ, Pepper M, Jenkins MK, Cleary PP. Robust antigen specific Th17 T cell response to Group A streptococcus is dependent on IL-6 and intranasal route of infection. PLoS Pathog. 2011;7(9):e1002252. doi: https://doi.org/10.1371/journal.ppat.1002252.CrossRefPubMedPubMedCentralGoogle Scholar
- 12.Sigurdardottir T, Björck V, Herwald H, Mörgelin M, Rutardottir S, Törnebrant J, Bodelsson M. M1 protein from Streptococcus pyogenes induces nitric oxide-mediated vascular hyporesponsiveness to phenylephrine: involvement of Toll-like receptor activation. Shock. 2010;34(1):98-104.CrossRefPubMedGoogle Scholar