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Bulletin of Experimental Biology and Medicine

, Volume 164, Issue 3, pp 347–350 | Cite as

Inactivation of M111 Protein Gene Modifies Streptococcus Pyogenes Interactions with Mouse Macrophages In Vitro

  • M. A. Suvorova
  • T. A. Kramskaya
  • A. N. Suvorov
  • E. P. Kiseleva
GENETICS
  • 22 Downloads

Immunomodulatory properties of S. pyogenes protein M111 were studied on the model of Gurov strain and its isogenic mutant not expressing M protein. Mouse resident peritoneal macrophages were incubated with bacteria and generation of nitroxide and superoxide anions and production of IL-6, IL-10, and IL-17 were evaluated. Protein M111 modified macrophage response: it exhibited antiphagocytic activity, prevented ROS formation, and stimulated the production of anti-inflammatory cytokine IL-10. The results suggested that this protein could serve in the bacteria as a factor suppressing the host defense forces and promoting the realization of the strategy beneficial for pathogens — escape from the host immune defense.

Key Words

Streptococcus pyogenes M protein macrophages superoxide radicals cytokines 

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References

  1. 1.
    Kiseleva EP, Puzyreva VP, Ogurtsov RP, Kovaleva IG. Effect of hyperlipidemia on functional activity of peritoneal macrophages in CBA and C57Bl/6 mice. Bull. Exp. Biol. Med. 2002;134(3):288-290.CrossRefPubMedGoogle Scholar
  2. 2.
    Suvorova MA, Tsapieva AN, Duplik NV, Kramskaya TA, Grabovskaya KB, Kiseleva EP, Chereshnev VA, Suvorov AN. Construction of a Streptococcus strain mutant by M protein gene. Med. Akad. Zh. 2016;16(4):235-236. Russian.Google Scholar
  3. 3.
    Areschoug T, Waldemarsson J, Gordon S. Evasion of macrophage scavenger receptor A-mediated recognition by pathogenic streptococci. Eur. J. Immunol. 2008;38(11):3068-3079.CrossRefPubMedGoogle Scholar
  4. 4.
    Courtney HS, Hasty DL, Dale JB. Molecular mechanisms of adhesion, colonization and invasion of group A streptococci. Ann. Med. 2002;34(2):77-87.CrossRefPubMedGoogle Scholar
  5. 5.
    Cunningham MW. Pathogenesis of group A streptococcal infections. Clin. Microbiol. Rev. 2000;13(3):470-511.CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Dileepan T, Linehan JL, Moon JJ, Pepper M, Jenkins MK, Cleary PP. Robust antigen specific Th17 T cell response to Group A streptococcus is dependent on IL-6 and intranasal route of infection. PLoS Pathog. 2011;7(9):e1002252. doi:  https://doi.org/10.1371/journal.ppat.1002252.CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Goldmann O, Sastalla I, Wos-Oxley M, Rohde M, Medina E. Streptococcus pyogenes induces oncosis in macrophages through the activation of an inflammatory programmed cell death pathway. Cell. Microbiol. 2009;11(1):138-155.CrossRefPubMedGoogle Scholar
  8. 8.
    Påhlman LI, Mörgelin M, Eckert J, Johansson L, Russell W, Riesbeck K, Soehnlein O, Lindbom L, Norrby-Teglund A, Schumann RR, Björck L, Herwald H. Streptococcal M protein: a multipotent and powerful inducer of inflammation. J. Immunol. 2006;177(2):1221-1228.CrossRefPubMedGoogle Scholar
  9. 9.
    Podbielski A, Schnitzler N, Beyhs P, Boyle MD. M-related protein (Mrp) contributes to group A streptococcal resistance to phagocytosis to human granulocytes. Mol. Microbiol. 1996;19(3):429-441.CrossRefPubMedGoogle Scholar
  10. 10.
    Price JD, Schaumburg J, Sandin C, Atkinson JP, Lindahl G, Kemper C. Induction of a regulatory phenotype in human CD4+ T cells by streptococcal M protein. J. Immunol. 2005;175(2):677-684.CrossRefPubMedGoogle Scholar
  11. 11.
    Sharma N, Toor D. Interleukin-10: role in increasing susceptibility and pathogenesis of rheumatic fever/rheumatic heart disease. Cytokine. 2017;90:169-176.CrossRefPubMedGoogle Scholar
  12. 12.
    Sigurdardottir T, Björck V, Herwald H, Mörgelin M, Rutardottir S, Törnebrant J, Bodelsson M. M1 protein from Streptococcus pyogenes induces nitric oxide-mediated vascular hyporesponsiveness to phenylephrine: involvement of Toll-like receptor activation. Shock. 2010;34(1):98-104.CrossRefPubMedGoogle Scholar
  13. 13.
    Staali L, Bauer S, Mörgelin M, Björck L, Tapper H. Streptococcus pyogenes bacteria modulate membrane traffic in human neutrophils and selectively inhibit azurophilic granule fusion with phagosomes. Cell. Microbiol. 2006;8(4):690-703.CrossRefPubMedGoogle Scholar
  14. 14.
    Steer AC, Law I, Matatolu L, Beall BW, Carapetis JR. Global emm type distribution of group A streptococci: systematic review and implications for vaccine development. Lancet Infect. Dis. 2009;9(10):611-616.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • M. A. Suvorova
    • 1
  • T. A. Kramskaya
    • 1
  • A. N. Suvorov
    • 1
  • E. P. Kiseleva
    • 1
    • 2
  1. 1.Institute of Experimental MedicineSt. PetersburgRussia
  2. 2.I. I. Mechnikov North-Western State Medical UniversitySt. PetersburgRussia

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