Bulletin of Experimental Biology and Medicine

, Volume 164, Issue 3, pp 334–338 | Cite as

Diagnostics of Early Changes in the Immune System Due to Low Concentration of N-Nitrosamines in the Blood

  • N. V. Zaitseva
  • T. S. Ulanova
  • O. V. Dolgikh
  • T. V. Nurislamova
  • O. A. Mal’tseva

The concentration of N-nitrosamines (N-nitrosodimethylamine and N-nitrosodiethylamine) was measured in blood samples from children after consumption of drinking water with high content of nitrates (main group) or water meeting health standards (reference group). N-nitrosodimethylamine level in the blood from children of the main group differed from that in the reference group by 2.6 times (0.00026±0.00012 and 0.0001±0.00092 mg/dm3, respectively; p<0.05). The specific immune response to N-nitrosodimethylamine exposure was manifested in an increase in the level of specific serum IgG (2 times higher than that in the reference group). An increase in the specific sensitivity to N-nitrosodimethylamine (by the criterion of IgG) was observed in 60.7% subjects. A correlation was found between an increase in the level of IgG to N-nitrosodimethylamine and rise in the concentration of N-nitrosodimethylamine in the blood (R 2 =0.35; p=0.021). Under these conditions the spontaneous and induced production of arachidonic acid metabolites (leukotrienes) increased by 2.1 times, while the expression of p53 transcription factor (responsible for oncosuppression) decreased by 1.9 times as compared to those in the reference group (p<0.05).

Key Words

N-nitrosamines chromatography-mass spectrometry p53 transcription factor IgG to nitrosamines 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Harmful C. Nitrogen-Containing Organic Compounds. Kurlyandskii BA, Filov VA, eds. St. Petersburg, 1992. Russian.Google Scholar
  2. 2.
    Galichiev SM, Makoeva LM, Dzhioev FK, Khaeva LKh. Potential pathways of endogenous formation of nitrosamines in gastric juice in vitro. Izv. Samarskogo Nauch. Tsentra Ross. Akad. Nauk. 2011;13(1-7):1678-1680. Russian.Google Scholar
  3. 3.
    Unitsky AP. Drinking water nitrates and nitrites as a carcinogenie risk factor. Gig. San. 2003(6):81-83. Russian.Google Scholar
  4. 4.
    Krasovsky GN, Plitman SI, Rogovets AI. Trends in water quality indices as a signal of population health hazard. Gig. San. 2003(6):26-27. Russian.Google Scholar
  5. 5.
    MR 4.2.0075-13. The List of Markers of Gene Polymorphism, Responsible for Features of Mutagenic Activity of Technogenic Chemical Factors. Moscow, 2013. Russian.Google Scholar
  6. 6.
    Mukhammadiyeva GF, Bakirov AB, Karimova LK, Valeyeva ET. Analysis of associations of polymorphic loci of a tumor suppressor gene tp53 with malignant neoplasms in glass fiber manufacturing workers. Gig. San. 2014;93(4):59-61.Google Scholar
  7. 7.
    Onishchenko GG, Zaitseva NV, May IV, Andreeva EE. Cluster systematization of the parameters of sanitary and epidemiological welfare of the population in the regions of the Russian Federation and the federal cities. Analiz Risky Zdorov’yu. 2016(1):4-14. Russian.Google Scholar
  8. 8.
    Zajtseva NV, Ulanova TS, Nurislamova TV, Popova NA, Maltseva OA, Ershova KS. Patent RU No. 2578026. Method for quantitative determination of N-nitrosodimethylamine and N-nitrosodiethylamine in blood by capillary gas chromatography. Bull. 8. Published March 20, 2016Google Scholar
  9. 9.
    Food and Health in Europe: a new basis for action. WHO regional publications. European series; No. 96. WHO, 2004.Google Scholar
  10. 10.
    IARC Monographs on the Evaluation of Carcinogenic Risks for Humans. Lyon, 1991. Vol. 52. P. 473.Google Scholar
  11. 11.
    Lee JM, Lee YC, Yang SY, Shi WL, Lee CJ, Luh SP, Chen CJ, Hsieh CY, Wu MT. Genetic polymorphisms of p53 and GSTP1, but not NAT2,are associated with susceptibility to squamous-cell carcinoma of the esophagus. Int. J. Cancer. 2000;89(5):458-464.CrossRefPubMedGoogle Scholar
  12. 12.
    McGregor D, Boobis A, Binaglia M, Botham P, Hoffstadt L, Hubbard S, Petry T, Riley A, Schwartz D, Hennes C. Guidance for the classification of carcinogens under the Globally Harmonised System of Classification and Labelling of Chemicals (GHS). Crit. Rev. Toxicol. 2010;40(3):245-285.CrossRefPubMedGoogle Scholar
  13. 13.
    Perera AMA. N-nitrosoamines. Chromatographic Analysis of the Environment. Nollet Leo ML, ed. CRC Press, 2006. P. 419-452.Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • N. V. Zaitseva
    • 1
  • T. S. Ulanova
    • 2
  • O. V. Dolgikh
    • 2
  • T. V. Nurislamova
    • 2
  • O. A. Mal’tseva
    • 1
  1. 1.Federal Scientific Center for Medical and Preventive Health Risk Management Technologies, Federal Service for Surveillance on Customers Rights Protection and Human WellbeingPermRussia
  2. 2.Perm National Research Polytechnic UniversityPermRussia

Personalised recommendations