Apolipoprotein A-I Stimulates Cell Proliferation in Bone Marrow Cell Culture
- 13 Downloads
Culturing of bone marrow cells in serum-free RPMI-1640 medium led to a decrease in the rate of DNA biosynthesis. Addition of HDL or their main protein component apolipoprotein A-I to the culture medium dose-dependently increased the rate of [3H]-thymidine incorporation into DNA. The maximum stimulation was achieved at HDL concentration of 80 μg/ml and apolipoprotein A-I concentration of 20 μg/ml. To identify the target-cells of apolipoprotein A-I, we used thymidine analogue 5-ethynyl-2’-deoxyuridine (EdU) that incorporates into cell DNA at the stage of replicative DNA synthesis (S phase) and can be detected by fluorescence microscopy. In bone marrow cell culture, apolipoprotein A-I stimulates the proliferation of monocyte (monoblasts, promonocytes) and granulocyte (myeloblasts, promyelocytes) progenitor cells, as well as bone marrow stromal cells.
Key Wordsbone marrow proliferation DNA synthesis plasma lipoproteins apolipoprotein A-I
Unable to display preview. Download preview PDF.
- 1.Goldberg ED, Dygai AM, Shakhov VP. Methods of Tissue Culture in Hematology. Tomsk, 1992. Russian.Google Scholar
- 2.Panin LE, Khar’kovskii AV, Usynin IF, Tuzikov FV, Tuzikova NA. Effect of tetrahydrocortisol-apolipoprotein A-I complex on protein biosynthesis in hepatocytes and on the secondary structure of eukaryotic DNA. Mol. Biol. 1999;33(4):596-601.Google Scholar
- 5.Pykhtina MB, Ivanov ID, Beklemishev AB. Development of effective ways of apolipoprotein A-I isolation from human plasma. Biofarmatsevt. Zh. 2012;4(6):37-45. Russian.Google Scholar
- 6.Andrew SM, Titus JA. Purification of immunoglobulin G. Curr. Protoc. Cell Biol. 2001. Chapter 16; Unit 16.3. doi: https://doi.org/10.1002/0471143030.cb1603s05.
- 12.Mills GL, Lane PA, Weech PK. The isolation and purification of plasma lipoproteins. Laboratory Techniques in Biochemistry and Molecular Biology: a Guidebook to Lipoprotein Technique. Burdon RH, Knippenberg PH, eds. Amsterdam, 1984. P. 18-116.Google Scholar
- 15.Zhou X, von Eckardstein A. Effect of HDL and apoAI on PGE2 production by monocyte-derived macrophages. J. Huazhong. Univ. Sci. Technolog. Med. Sci. 2002;22(4):270-272.Google Scholar