Bulletin of Experimental Biology and Medicine

, Volume 164, Issue 3, pp 308–311 | Cite as

Apolipoprotein A-I Stimulates Cell Proliferation in Bone Marrow Cell Culture

  • I. F. Usynin
  • A. N. Dudarev
  • A. Yu. Gorodetskaya
  • S. M. Miroshnichenko
  • T. A. Tkachenko
  • V. I. Tkachenko

Culturing of bone marrow cells in serum-free RPMI-1640 medium led to a decrease in the rate of DNA biosynthesis. Addition of HDL or their main protein component apolipoprotein A-I to the culture medium dose-dependently increased the rate of [3H]-thymidine incorporation into DNA. The maximum stimulation was achieved at HDL concentration of 80 μg/ml and apolipoprotein A-I concentration of 20 μg/ml. To identify the target-cells of apolipoprotein A-I, we used thymidine analogue 5-ethynyl-2’-deoxyuridine (EdU) that incorporates into cell DNA at the stage of replicative DNA synthesis (S phase) and can be detected by fluorescence microscopy. In bone marrow cell culture, apolipoprotein A-I stimulates the proliferation of monocyte (monoblasts, promonocytes) and granulocyte (myeloblasts, promyelocytes) progenitor cells, as well as bone marrow stromal cells.

Key Words

bone marrow proliferation DNA synthesis plasma lipoproteins apolipoprotein A-I 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • I. F. Usynin
    • 1
  • A. N. Dudarev
    • 1
  • A. Yu. Gorodetskaya
    • 1
  • S. M. Miroshnichenko
    • 1
  • T. A. Tkachenko
  • V. I. Tkachenko
    • 1
  1. 1.Research Institute of BiochemistryNovosibirskRussia

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