Bulletin of Experimental Biology and Medicine

, Volume 162, Issue 4, pp 578–582 | Cite as

Expression of Surface Molecules in Human Mesenchymal Stromal Cells Co-Cultured with Nucleated Umbilical Cord Blood Cells

  • Yu. A. Romanov
  • E. E. Balashova
  • N. E. Volgina
  • N. V. Kabaeva
  • T. N. Dugina
  • G. T. Sukhikh
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)

We studied the expression of different classes of surface molecules (CD13, CD29, CD40, CD44, CD54, CD71, CD73, CD80, CD86, CD90, CD105, CD106, CD146, HLA-I, and HLA-DR) in mesenchymal stromal cells from human umbilical cord and bone marrow during co-culturing with nucleated umbilical cord blood cells. Expression of the majority of surface markers in both types of mesenchymal stromal cells was stable and did not depend on the presence of the blood cells. Significant differences were found only for cell adhesion molecules CD54 (ICAM-1) and CD106 (VCAM-1) responsible for direct cell—cell contacts with leukocytes and only for bone marrow derived cells.

Key Words

mesenchymal stromal cells umbilical cord blood co-culture differentiation clusters flow cytometry 


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Yu. A. Romanov
    • 1
    • 3
  • E. E. Balashova
    • 3
  • N. E. Volgina
    • 2
  • N. V. Kabaeva
    • 1
  • T. N. Dugina
    • 3
  • G. T. Sukhikh
    • 2
  1. 1.Russian Cardiology Research-and-Production ComplexMinistry of Health of the Russian FederationMoscowRussia
  2. 2.V. I. Kulakov Research Center of Obstetrics, Gynecology, and PerinatologyMinistry of Health of the Russian FederationMoscowRussia
  3. 3.CryoCenter Cord Blood BankMoscowRussia

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