Skip to main content

Advertisement

SpringerLink
Log in

Heterogeneity of Retinal Pigment Epithelial Cells from Adult Human Eye in Different Culturing Systems

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

We studied the behavior of retinal pigment epithelial cells from adult human eye derived from different donors in different culturing systems: on plastic, in collagen gel, and on decellularized neural retina substrate. The cells diverge into two subpopulations similar by their morphology and behavior: one subpopulation migrated to the surface of the dense substrate and the other formed spheroid structures consisting of aggregated cells. This fact confirms the data on genetically-predetermined phenotypic heterogeneity of retinal pigment epithelium cells that should be taken into account when using these cells in tissue engineering.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Kuznetsova AV, Milyushina LA, Mikaelyan AS, Zinov’eva RD, Grigoryan EN, Aleksandrova MA. Dedifferentiation of retinal pigment epithelial cells from adult human eye in vitro. Mol. Med. 2010;(6):23-29.

  2. Alge CS, Suppmann S, Priglinger SG, Neubauer AS, May CA, Hauck S, Welge-Lussen U, Ueffing M, Kampik A. Comparative proteome analysis of native differentiated and cultured dedifferentiated human RPE cells. Invest. Ophthalmol. Vis. Sci. 2003;44(8):3629-3641.

    Article  PubMed  Google Scholar 

  3. Badylak SF, Freytes DO, Gilbert TW. Extracellular matrix as a biological scaffold material: structure and function. Acta Biomater. 2009;5(1):1-13.

    Article  CAS  PubMed  Google Scholar 

  4. Binder S. Scaffolds for retinal pigment epithelium (RPE) replacement therapy. Br. J. Ophthalmol. 2011;95(4):441-442. doi: 10.1136/bjo.2009.171926.

    Article  PubMed  Google Scholar 

  5. Burke JM, Hjelmeland LM. Mosaicism of the retinal pigment epithelium: seeing the small picture. Mol. Interv. 2005; 5(4):241-249.

    Article  PubMed  Google Scholar 

  6. Cong L, Sun D, Zhang Z, Jiao W, Rizzolo LJ, Peng S. A novel rabbit model for studying RPE transplantation. Invest. Ophthalmol. Vis. Sci. 2008;49(9):4115-4125.

    Article  PubMed  PubMed Central  Google Scholar 

  7. da Cruz L, Chen FK, Ahmado A, Greenwood J, Coffey P. RPE transplantation and its role in retinal disease. Prog. Retin. Eye Res. 2007;26(6):598-635.

    Article  PubMed  Google Scholar 

  8. Donato R. S100: a multigenic family of calcium-modulated proteins of the EF-hand type with intracellular and extracellular functional roles. Int. J. Biochem. Cell Biol. 2001;33(7):637-668.

    Article  CAS  PubMed  Google Scholar 

  9. Duda T, Koch KW, Venkataraman V, Lange C, Beyermann M, Sharma RK. Ca(2+) sensor S100beta-modulated sites of membrane guanylate cyclase in the photoreceptor-bipolar synapse. EMBO J. 2002;21(11):2547-2556.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Dunmire JJ, Bouhenni R, Hart ML, Wakim BT, Chomyk A.M, Scott S.E, Nakamura H, Edward D.P. Novel serum proteomic signatures in a non-human primate model of retinal injury. Mol. Vis. 2011;17:779-791.

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Fuchs U, Kivelä T, Tarkkanen A. Cytoskeleton in normal and reactive human retinal pigment epithelial cells. Invest. Ophthalmol. Vis. Sci. 1991;32(13):3178-3186.

    CAS  PubMed  Google Scholar 

  12. Grisanti S, Guidry C. Transdifferentiation of retinal pigment epithelial cells from epithelial to mesenchymal phenotype. Invest. Ophthalmol. Vis. Sci. 1995;36(2):391-405.

    CAS  PubMed  Google Scholar 

  13. Hernández M, Pearce-Kelling S.E, Rodriguez F.D, Aguirre G.D, Vecino E. Altered expression of retinal molecular markers in the canine RPE65 model of Leber congenital amaurosis. Invest. Ophthalmol. Vis Sci. 2010;51(12):6793-6802.

    Article  PubMed  PubMed Central  Google Scholar 

  14. Kuznetsova AV. Morphological and physiological characteristics of the native retinal pigment epithelium in vertebrate animals and human. Biol. Bull. Rev. 2014;4(2):71-85.

    Article  Google Scholar 

  15. Kuznetsova AV, Grigoryan EN, Aleksandrova MA. Human adult retinal pigment epithelial cells as potential cell source for retina recovery. Cell Tssue Biol. 2011;5(5):495-502.

    Article  Google Scholar 

  16. Milyushina LA, Kuznetsova AV, Grigoryan EN, Aleksandrova MA. Phenotypic plasticity of retinal pigment epithelial cells from adult human eye in vitro. Bull. Exp. Biol. Med. 2011;151(4):506-511.

    Article  CAS  PubMed  Google Scholar 

  17. Milyushina LA, Verdiev BI, Kuznetsova AV, Aleksandrova MA. Expression of multipotent and retinal markers in pigment epithelium of adult human in vitro. Bull. Exp. Biol. Med. 2012;153(1):157-162.

    Article  CAS  PubMed  Google Scholar 

  18. Qin S, Rodrigues GA. Progress and perspectives on the role of RPE cell inflammatory responses in thedevelopment of age-related macular degeneration. J. Inflamm. Res. 2008;1:49-65.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Sheridan C, Hiscott P, Grierson I. Retinal pigment epithelium differentiation and dedifferentiation. Vitreo-retinal Surgery. Kirchhof B, Wong D. New York, 2005. P. 101-119.

  20. Stanzel BV, Espana EM, Grueterich M, Kawakita T, Parel JM, Tseng SC, Binder S. Amniotic membrane maintains the phenotype of rabbit retinal pigment epithelial cellsin culture. Exp. Eye Res. 2005;80(1):103-112.

    Article  CAS  PubMed  Google Scholar 

  21. Tsonis PA, Jang W, Del Rio-Tsonis K, Eguchi G. A unique aged human retinal pigmented epithelial cell line useful for studying lens differentiation in vitro. Int. J. Dev. Biol. 2001;45(5-6):753-758.

    CAS  PubMed  Google Scholar 

  22. van Meurs JC, Van Den Biesen PR. Autologous retinal pigment epithelium and choroid translocation in patients with exudative age-related macular degeneration: short-term follow-up. Am. J. Ophthalmol. 2003;136(4):688-695.

    Article  PubMed  Google Scholar 

  23. Voroteliak EA, Shikhverdieva ASh, Vasil’ev AV, Terskikh VV. Simulation of the migration process of human epidermal keratinocytes over three-dimensional collagen gel. Izv. Akad. Nauk. Ser. Biol. 2002;(1):30-37.

Download references

Author information

Authors and Affiliations

  1. N. K. Kol’tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia

    A. V. Kuznetsova & M. A. Aleksandrova

Authors
  1. A. V. Kuznetsova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. A. Aleksandrova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. V. Kuznetsova.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 260-269, October, 2016

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kuznetsova, A.V., Aleksandrova, M.A. Heterogeneity of Retinal Pigment Epithelial Cells from Adult Human Eye in Different Culturing Systems. Bull Exp Biol Med 162, 569–577 (2017). https://doi.org/10.1007/s10517-017-3661-x

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-017-3661-x

Key Words

Search

Navigation