Bulletin of Experimental Biology and Medicine

, Volume 162, Issue 4, pp 558–562 | Cite as

Visualization of Mesenchymal Stromal Cells in 2Dand 3D-Cultures by Scanning Electron Microscopy with Lanthanide Contrasting

  • I. A. Novikov
  • I. V. Vakhrushev
  • E. N. Antonov
  • K. N. Yarygin
  • A. M. Subbot
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • 51 Downloads

Mesenchymal stromal cells from deciduous teeth in 2D- and 3D-cultures on culture plastic, silicate glass, porous polystyrene, and experimental polylactoglycolide matrices were visualized by scanning electron microscopy with lanthanide contrasting. Supravital staining of cell cultures with a lanthanide-based dye (neodymium chloride) preserved normal cell morphology and allowed assessment of the matrix properties of the carriers. The developed approach can be used for the development of biomaterials for tissue engineering.

Key Words

scanning electron microscopy lanthanide mesenchymal stromal cells tissue engineering 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • I. A. Novikov
    • 1
  • I. V. Vakhrushev
    • 2
  • E. N. Antonov
    • 3
  • K. N. Yarygin
    • 2
  • A. M. Subbot
    • 1
  1. 1.Institute of Eye DiseasesMoscowRussia
  2. 2.V. N. Orekhovich Research Institute of Biomedical ChemistryMoscowRussia
  3. 3.Institute on Laser and Information TechnologiesRussian Academy of SciencesMoscowRussia

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