Characteristics of Multipotent Mesenchymal Stromal Cells Isolated from the Endometrium and Endometriosis Lesions of Women with Malformations of the Internal Reproductive Organs
- 85 Downloads
We isolated and characterized cell cultures from eutopic endometrium and endometriotic lesions of women with malformations of the internal reproductive organs. The cells had fibroblast-like shape and intensively expressed CD90, CD73, CD105, CD44, CD146, and CD117 and were capable of induced adipogenic and osteogenic differentiation in vitro. The obtained cultures exhibited properties of multipotent mesenchymal stromal cells; at the same time, they demonstrated in vitro immunophenotypic differences from cell cultures of eutopic and ectopic endometrium of women without developmental abnormalities, which suggests their functional difference. The cells from eutopic endometrium and from ectopic endometriotic lesions can be used as the model for studying of the etiology and pathogenesis of endometriosis and for testing new drugs for this specific group of patients. Markers CD90 and CD117 were identified as promising molecules for the development of minimally invasive diagnostics of endometriosis based on cell cultures from eutopic endometrium.
Key Wordsendometriosis endometrium multipotent mesenchymal stromal cells immunophenotype malformations of the internal reproductive organs
Unable to display preview. Download preview PDF.
- 1.Adamyan LV, Kulakov VI, Khashukoeva AZ. Malformations of the uterus and vagina. Moscow, 1998. Russian.Google Scholar
- 2.Adamyan LV, Spitsin VA, Andreeva EN. Genetic aspects of gynecological diseases. Moscow, 1998. Russian.Google Scholar
- 4.Maslova MA, Smol’nikova VY, Savilova AM, Burmenskaya OV, Bystritskii AA, Tabolova VK, Korneeva IE, Demura TA, Donnikov AE. Profiles of mRNA expression for genes involved in implantation, early and middle phases of secretion stage in human endometrium. Bull. Exp. Biol. Med. 2015;158(6):781-784.CrossRefPubMedGoogle Scholar
- 9.Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop Dj, Horwitz E. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006;8(4):315-317.CrossRefPubMedGoogle Scholar
- 13.Jazedje T, Perin PM, Czeresnia CE, Maluf M, Halpern S, Secco M, Bueno DF, Vieira NM, Zucconi E, Zatz M. Human fallopian tube: a new source of multipotent adult mesenchymal stem cells discarded in surgical procedures. J. Transl. Med. 2009;7:46. doi: 10.1186/1479-5876-7-46.CrossRefPubMedPubMedCentralGoogle Scholar
- 14.Klemmt PA, Carver JG, Koninckx P, McVeigh EJ, Mardon HJ. Endometrial cells from women with endometriosis have increased adhesion and proliferative capacity in response to extracellular matrix components: towards a mechanistic model for endometriosis progression. Hum. Reprod. 2007;22(12):3139-3147.CrossRefPubMedGoogle Scholar
- 19.Russell KC, Phinney DG, Lacey MR, Barrilleaux BL, Meyertholen KE, O’Connor KC. In vitro high-capacity assay to quantify the clonal heterogeneity in trilineage potential of mesenchymal stem cells reveals a complex hierarchy of lineage commitment. Stem Cells. 2010;28(4):788-798.CrossRefPubMedGoogle Scholar
- 20.Schrobback K, Wrobel J, Hutmacher DW, Woodfield TB, Klein TJ. Stage-specific embryonic antigen-4 is not a marker for chondrogenic and osteogenic potential in cultured chondrocytes and mesenchymal progenitor cells. Tissue Eng. Part A. 2013;19(11-12):1316-1326.CrossRefPubMedPubMedCentralGoogle Scholar