Bulletin of Experimental Biology and Medicine

, Volume 162, Issue 4, pp 528–533 | Cite as

Human Umbilical Cord Blood Serum: Effective Substitute of Fetal Bovine Serum for Culturing of Human Multipotent Mesenchymal Stromal Cells

  • Yu. A. Romanov
  • E. E. Balashova
  • N. E. Volgina
  • N. V. Kabaeva
  • T. N. Dugina
  • G. T. Sukhikh

Optimal conditions for culturing of multipotent mesenchymal stromal cells in the presence of pooled umbilical cord blood serum were determined. It was found that umbilical cord blood serum in a concentration range of 1-10% effectively supported high viability and proliferative activity of cells with unaltered phenotype and preserved multilineage differentiation capacity. The proposed approach allows avoiding the use of xenogenic animal sera for culturing of multipotent mesenchymal stromal cells and creates prerequisites for designing and manufacturing safe cellular and/or acellular products for medical purposes.

Key Words

multipotent mesenchymal stromal cells umbilical cord blood serum proliferation phenotype differentiation 


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Yu. A. Romanov
    • 1
    • 3
  • E. E. Balashova
    • 1
    • 3
  • N. E. Volgina
    • 2
  • N. V. Kabaeva
    • 1
  • T. N. Dugina
    • 3
  • G. T. Sukhikh
    • 2
  1. 1.Russian Cardiology Research-and-Production ComplexMinistry of Health of the Russian FederationMoscowRussia
  2. 2.V. I. Kulakov Research Center of Obstetrics, Gynecology, and PerinatologyMinistry of Health of the Russian FederationMoscowRussia
  3. 3.CryoCenter Cord Blood BankMoscowRussia

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