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Bulletin of Experimental Biology and Medicine

, Volume 162, Issue 4, pp 501–506 | Cite as

Conditions for Collection of Placental Tissue Samples for Culturing of Multipotent Mesenchymal Stromal Cells

  • N. V. Nizyaeva
  • M. N. Nagovitsyna
  • G. V. Kulikova
  • U. N. Tumanova
  • R. A. Poltavtseva
  • I. A. Fedorova
  • M. N. Yushina
  • S. V. Pavlovich
  • A. I. Shchyogolev
MORPHOLOGY AND PATHOMORPHOLOGY

Placentas from women aged 25-32 years with normal course of gestation were studied. It is essential to stick to certain methodological approaches for preparing viable multipotent mesenchymal stromal cell culture and to carry out morphological (macro and micro) evaluation of the chorionic villi, umbilical cords, and placentas. At stage I of the study, patients’ histories, labor course, and examinations of the newborns should be analyzed to exclude women with genital and extragenital diseases. At stage II, it is essential to stick to special regulations and methods for collection of specimens of the cord, amnion, and placental tissue proper. Histological control of the placental structures collected for multipotent mesenchymal stromal cell culturing is obligatory.

Key Words

amnion cell culturing multipotent mesenchymal stromal cells placenta umbilical cord 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • N. V. Nizyaeva
    • 1
  • M. N. Nagovitsyna
    • 1
  • G. V. Kulikova
    • 1
  • U. N. Tumanova
    • 1
  • R. A. Poltavtseva
    • 1
  • I. A. Fedorova
    • 1
  • M. N. Yushina
    • 1
  • S. V. Pavlovich
    • 1
  • A. I. Shchyogolev
    • 1
  1. 1.V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatologythe Ministry of Health of the Russian FederationMoscowRussia

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