Bulletin of Experimental Biology and Medicine

, Volume 162, Issue 4, pp 496–500 | Cite as

Myeloid Precursors in the Bone Marrow of Mice after a 30-Day Space Mission on a Bion-M1 Biosatellite

  • E. V. Sotnezova
  • E. A. Markina
  • E. R. Andreeva
  • L. B. Buravkova

The content of myeloid stem CFU in bone marrow karyocytes from the tibial bone of C57Bl/6 mice was evaluated after a 30-day Bion-M1 pace flight/ground control experiment and subsequent 7-day recovery period. After the space flight, we observed a significant decrease in the number of erythroid progenitors in the bone marrow, including common myeloid precursor — granulocyte, erythrocyte, monocyte/macrophage, megakaryocyte CFU. After 7-day readaptation, CFU level in flight animals did not recover completely. In the ground control, the count of erythroid burst-forming units was higher than in vivarium animals. Comparison of the changes observed in fight and ground experiments demonstrated effects associated space flight factors and manifesting in suppression of the bone marrow erythropoiesis.

Key Words

Bion-M1 biosatellite C57Bl/6 mice bone marrow karyocytes hemopoietic colony-forming units 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Andreeva ER, Goncharova EA, Gornostaeva AN, Grigorieva OV, Buravkova LB. Bone marrow mononuclear cells from murine tibia after the space flight on biosatellite “Bion-M1”. Aviakosm. Ekol. Med. 2014;48(2):5-11. Russian.Google Scholar
  2. 2.
    Andreev-Andrievsky AA, Shenkman BS, Popova AS, Dolguikh ON, Anokhin KV, Soldatov PE, Ilyin EA, Sychev VN. Experimental studies with mice on the program of the biosatellite Bion-M1 mission. Aviakosm. Ekol. Med. 2014;48(1):14-27. Russian.Google Scholar
  3. 3.
    Ivanova SM. Blood system under conditions of space flights and after their completion. Mir space station. Space Biology and Medicine. Vol. 2. Grigor’eva AI, ed. Moscow, 2002. P. 159-160. Russian.Google Scholar
  4. 4.
    Ivanova SM, Yarlykova YuV, Labetskaya OI, Karashtin VV, Levina AA, Shishkanova ZG, Tsybul’skaya MM, Kozinets GI. The effect of spaceflight factors on human peripheral red blood. Aviakosm. Ekol. Med. 1998;32(1):35-40. Russian.Google Scholar
  5. 5.
    Kalandarova MP, Polyakov VV, Goncharov IB, Tikhonova LYu. Hematological parameters in astronauts during space flight. Aviakosm. Ekol. Med. 1991;25(6):11-14. Russian.Google Scholar
  6. 6.
    Serova LV, Chel’naya NA, Ivanova SYa. Comparative analysis of the effects of micro- and hypergravity on erythroid hemopoiesis in male and female mammals. Aviakosm. Ekol. Med. 1993;27(1):54-59. Russian.Google Scholar
  7. 7.
    Sychev VN, Ilyin EA, Yarmanova EN, Rakov DV, Ushakov IB, Kirilin AN, Orlov OI, Grigoriev AI. The Bion-M1 project: overview and first results. Aviakosm. Ekol. Med. 2014;48(1):7-14. Russian.Google Scholar
  8. 8.
    Blaber EA, Dvorochkin N, Torres ML, Yousuf R, Burns BP, Globus RK, Almeida EA. Mechanical unloading of bone in microgravity reduces mesenchymal and hematopoietic stem cellmediated tissue regeneration. Stem Cell Res. 2014;13(2):181-201.CrossRefPubMedGoogle Scholar
  9. 9.
    Gridley DS, Nelson GA, Peters LL, Kostenuik PJ, Bateman TA, Morony S, Stodieck LS, Lacey DL, Simske SJ, Pecaut MJ. Genetic models in applied physiology: selected contribution: effects of spaceflight on immunity in the C57BL/6 mouse. II. Activation, cytokines, erythrocytes, and platelets. J. Appl. Physiol. (1985). 2003;94(5):2095-2103.Google Scholar
  10. 10.
    Meirelles Lda S, Nardi NB. Murine marrow-derived mesenchymal stem cell: isolation, in vitro expansion, and characterization. Br. J. Haematol. 2003;123(4):702-711.CrossRefPubMedGoogle Scholar
  11. 11.
    Vacek A, Michurina TV, Serova LV, Rotkovská D, Bartonícková A. Decrease in the number of progenitors of erythrocytes (BFUe, CFUe), granulocytes and macrophages (GM-CFC) in bone marrow of rats after a 14-day flight onboard the Cosmos-2044 Biosatellite. Folia Biol. (Praha). 1991;37(1):35-41.Google Scholar
  12. 12.
    Udden MM, Driscoll TB, Gibson LA, Patton CS, Pickett MH, Jones JB, Nachtman R, Allebban Z, Ichiki AT, Lange RD. Blood volume and erythropoiesis in the rat during spaceflight. Aviat. Space Environ. Med. 1995;66(6):557-561.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • E. V. Sotnezova
    • 1
  • E. A. Markina
    • 1
  • E. R. Andreeva
    • 1
  • L. B. Buravkova
    • 1
  1. 1.Institute of Biomedical Problems, Russian Academy of SciencesMoscowRussia

Personalised recommendations