Proliferative and Differentiation Potential of Multipotent Mesenchymal Stem Cells Cultured on Biocompatible Polymer Scaffolds with Various Physicochemical Characteristics
- 66 Downloads
Biocompatibility of film and fibrous scaffolds from polylactide-based polymers and the relationship between their architecture and the functional characteristics of mesenchymal stem cells were studied. Cell culturing on polylactide-based film and fibrous matrixes did not deteriorate cell morphology and their proliferation and differentiation capacities. The rate of cell proliferation and penetration in microporous 3D matrices with the same porosity parameters and pore size depended on their spatial organization. The above materials can be used as scaffolds for mesenchymal stem cells for creation of tissue engineering implants. The scaffold size and structure should be determined by the defects in the organs in which the regeneration processes have to be stimulated.
Key Wordsmesenchymal stem cells polylactide scaffolds differentiation regenerative medicine
Unable to display preview. Download preview PDF.
- 1.Bogdanova OI, Sedush NG, Ovchinnikova TN, Belousov SI, Polyakov DK, Chvalun SN. Polylactide, a biodegradable, biocompatible polymer based on plant raw materials. Ekol. Prom. Rossii. 2010;(Spets. Vypusk 5):18-23. Russian.Google Scholar
- 2.Sarkisov DS, Perov YuM. Microscopic Technique. Moscow, 1996;16-20. Russian.Google Scholar
- 3.Shved YuA, Kukhareva LV, Zorin IM, Solovjov AYu, Blinova MI, Bilibin AYu, Pinaev GP. Elaboration of biodegradable polymer substrate for cultivation of human dermal fibroblasts. Tsitologiya. 2006;48(2):161-168. Russian.Google Scholar
- 5.Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop Dj, Horwitz E. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006;8(4):315-317.CrossRefPubMedGoogle Scholar
- 11.Schusser S, Menzel S, Bäcker M, Leinhos M, Poghossian A, Wagner P, Schöning MJ. Degradation of thin poly(lactic acid) films: Characterization by capacitance—voltage, atomic force microscopy, scanning electron microscopy and contact-angle measurements. Electrochim. Acta. 2013;113):779-778.Google Scholar