Bulletin of Experimental Biology and Medicine

, Volume 162, Issue 2, pp 273–276 | Cite as

Modern Approaches to Studies of New Osteogenic Biomaterials on the Model of Regeneration of Critical-Size Cranial Defects in Rats

  • M. S. Bartov
  • A. V. Gromov
  • M. S. Poponova
  • D. M. Savina
  • K. E. Nikitin
  • T. M. Grunina
  • V. N. Manskikh
  • O. A. Gra
  • V. G. Lunin
  • A. S. Karyagina
  • A. L. Gintsburg
Article

Osteoinductive characteristics of new osteoplastic materials based on demineralized bone matrix of xenogenic origin with high and controlled degree of purification were studied on the model of regeneration of critical-size cranial defects in rats using modern approaches, including histological analysis, evaluation of morphological parameters of the bone tissue obtained by micro-computed tomography, and estimation of bone tissue growth rate using in vivo fluorochrome label. Demineralized bone matrix and, to a much greater extent, its activated form containing modified recombinant growth factor rhBMP-2 with high content of the dimeric form exhibited osteoinductive activity.

Key Words

demineralized bone matrix BMP-2 osteoinductive activity bone tissue regeneration defect of critical size 

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References

  1. 1.
    Bartov MS, Karyagina AS, Gromov AV, Mishina DM, Trunova GV, Sidorova EI, Andreeva EV, Donchenko SV, Mukhametov FF, Mukhametov UF, Mirgazizov MZ, Mirgazizov AM, Khafizov RG, Lunin VG, Filippova NE, Gintsburg AL. New generation osteoplastic materials GAMALANT, containing growth and regeneration protein factors. Kafedra Travmatologii Ortopedii. 2012;(2):21-25. Russian.Google Scholar
  2. 2.
    Grigor’yan AS, Toporkova AK.Problems of Implant Integration into the Bone Tissue (Theoretical Aspects). Moscow, 2007. Russian.Google Scholar
  3. 3.
    Gromov AV, Nikitin KE, Karpova TA, Bartov MS, Mishina DM, Subbotina ME, Shevliagina NV, Sergienkov MA, Soboleva LA, Kotnova AP, Sharapova NE, Semikhin AS, Didenko LV, Kariagina AS, Lunin VG. Development of a method for obtaining of dimineralized bone matrix with high residual content of native bone tissue growth factors. Biotechnology in Russia. 2012;(5):66-75.Google Scholar
  4. 4.
    Sharapova NE, Kotnova AP, Galushkina ZM, Lavrova NV, Poletaeva NN, Tukhvatulin AE, Semikhin AS, Gromov AV, Soboleva LA, Ershova AS, Sergienko OV, Lunin VG, Karyagina AS, Zaitsev VV. Production of the recombinant human bone morphogenetic protein-2 in Escherichia coli and testing of its biological activity in vitro and in vivo. Molecular Biology. 2010;44(6):923-930.CrossRefGoogle Scholar
  5. 5.
    Boerckel JD, Kolambkar YM, Dupont KM, Uhrig BA, Phelps EA, Stevens HY, García AJ, Guldberg RE. Effects of protein dose and delivery system on BMP-mediated bone regeneration. Biomaterials. 2011;32(22):5241-5251.CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Cowan CM, Aghaloo T, Chou YF, Walder B, Zhang X, Soo C, Ting K, Wu B. MicroCT evaluation of three-dimensional mineralization in response to BMP-2 doses in vitro and in critical sized rat calvarial defects. Tissue Eng. 2007;13(3):501-512.CrossRefPubMedGoogle Scholar
  7. 7.
    Drosos GI, Kazakos KI, Kouzoumpasis P, Verettas DA. Safety and efficacy of commercially available demineralised bone matrix preparations: a critical review of clinical studies. Injury. 2007;38(Suppl. 4):S13-S21.CrossRefPubMedGoogle Scholar
  8. 8.
    Hollinger JO, Kleinschmidt JC. The critical size defect as an experimental model to test bone repair materials. J. Craniofac. Surg. 1990;1(1):60-68.CrossRefPubMedGoogle Scholar
  9. 9.
    Russell JL, Block JE. Clinical utility of demineralized bone matrix for osseous defects, arthrodesis, and reconstruction: impact of processing techniques and study methodology. Orthopedics. 1999;22(5):524-531.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • M. S. Bartov
    • 1
  • A. V. Gromov
    • 1
  • M. S. Poponova
    • 1
  • D. M. Savina
    • 1
  • K. E. Nikitin
    • 1
  • T. M. Grunina
    • 1
  • V. N. Manskikh
    • 2
  • O. A. Gra
    • 1
  • V. G. Lunin
    • 1
  • A. S. Karyagina
    • 1
    • 2
  • A. L. Gintsburg
    • 1
  1. 1.N. F. Gamaleya Federal Research Center of Epidemiology and MicrobiologyMinistry of Health of the Russian FederationMoscowRussia
  2. 2.A. N. Belozersky Institute of Physico-Chemical BiologyM. V. Lomonosov Moscow State UniversityMoscowRussia

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