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Microeukaryote community and the nutritional composition of the biofloc during Nile tilapia culture in water-reusing biofloc systems

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Abstract

A 25-week experiment was conducted to investigate the changes in the microeukaryote community (MEC) and nutritional composition of the biofloc during Nile tilapia culture in water-reusing biofloc systems. The study evaluated two treatments: tilapia culture in biofloc (TB) and tilapia culture in biofloc with reuse water (RWTB). Each of the treatments was evaluated using a main culture tank (3 m3) and three experimental tanks (0.2 m3). The biofloc samples were revised with an inverted microscope and the groups of microorganisms and genera of the MEC were recorded. Total abundance of microorganisms (TAM), number of accumulated genera, ecological indices and water quality were evaluated. The MEC of both treatments consisted of microalgae, ciliates, flagellates, amoebas, rotifers, nematodes and annelids; the number of accumulated genera and TAM in RWTB (74 genera, 161.52 ± 80.60 org mL−1) was significantly greater than in TB (63 genera, 139.54 ± 81.39 org mL−1). The TAM in TB and RWTB varied in relation to the temperature, sedimentable solids, NH4-N and NH3-N. The dominance index observed in the RWTB group (0.59) was statistically greater than in the TB group (0.57) during the first 2 weeks of the study. The nutritional composition of the biofloc varied with time; at the end of the study, an increase in the percentage of protein (47%) and a reduction in the percentage of lipids (2%) was observed when compared with values recorded at the beginning of the study. The results suggest that both the richness and TAM of the MEC increase in water-reusing biofloc systems and that the biochemical constitution of the microorganisms which constitute the MEC affects the proximal composition of the biofloc.

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Abbreviations

MEC:

Microeukaryote community

TB:

Tilapia culture in biofloc

RWTB:

Tilapia culture in biofloc with reuse water

TAM:

Total abundance of microorganisms

BFT:

Biofloc technology

SS:

Settleable solids

T:

Temperature

DO:

Dissolved oxygen

TDS:

Total dissolved salts

GH:

General hardness

KH:

Carbonate hardness

NFE:

Nitrogen-free extract

PC1:

Principal component 1

PC2:

Principal component 2

References

  • Abolafia J (2006) Order Rhabditida: Suborder Rhabditina. In: Eyualem-Abebe AI, Traunspurger W (eds) Freshwater nematodes: ecology and taxonomy. CABI Publishing, Oxford

    Google Scholar 

  • AOAC (1990) Official methods of analysis of the association of official analytical chemists, 15th edn. Association of Official Analytical Chemists, Arlington, VA

    Google Scholar 

  • APHA (1995) Standard methods for examination of water and wastewater. American Public Health Association, Washington D.C

    Google Scholar 

  • Asaduzzaman M, Rahman MM, Azim ME, Islam MA, Wahab MA, Verdegem MCJ, Verreth JAJ (2010) Effects of C/N ratio and substrate addition on natural food communities in freshwater prawn monoculture ponds. Aquaculture 306:127–136

    Article  CAS  Google Scholar 

  • Avnimelech Y (1999) Carbon/nitrogen ratio as a control element in aquaculture systems. Aquaculture 176:227–235

    Article  CAS  Google Scholar 

  • Azim ME, Little DC, Bron JE (2008) Microbial protein production in activated suspension tanks manipulating C:N ratio in feed and the implications for fish culture. Bioresour Technol 99:3590–3599

    Article  CAS  PubMed  Google Scholar 

  • Bellinger EG, Sigee DC (2015) Freshwater algae: identification, enumeration and use as bioindicators, 2nd edn. Willey Blackwell, Oxford

    Google Scholar 

  • Browdy CL (2014) Shrimp biofloc technologies, feeds and gut health. In: Browdy CL, Hargreaves J, Tung H, Avnimelech Y (eds) Proceedings of the Biofloc Technology and Shrimp Disease Workshop. Ho Chi Minh, 9–10 Dec 2013

  • Colwell RK (2013) EstimateS: statistical estimation of species richness and shared species from samples. Available via DIALOG. http://viceroy.eeb.uconn.edu/estimates/ Cited 22 Oct 2016

  • De Schryver P, Crab R, Defoirdt T et al (2008) The basics of bio-flocs technology: the added value for aquaculture. Aquaculture 277:125–137

    Article  CAS  Google Scholar 

  • Ebeling JM, Timmons MB, Bisogni JJ (2006) Understanding photoautotrophic, autotrophic, and heterotrophic bacterial based systems using basic water quality parameters. In: Rakestraw TT, Douglas LS, Marsh L, Granata L, Correa A, Flick GJ (eds) Proceedings of the 6th International Conference on Recirculation Aquaculture. Roanoke, VA, 20–22 July 2006

  • Emerenciano M, Ballester C, Cavalli R et al (2011) Effect of biofloc technology (BFT) on the early postlarval stage of pink shrimp Farfantepenaeus paulensis: growth performance, floc composition and salinity stress tolerance. Aquac Int 19:891–901

    Article  Google Scholar 

  • Emerenciano M, Martínez-Córdova L, Martínez-Porchas M et al (2017) Biofloc Technology (BFT): A Tool for Water Quality Management in Aquaculture. In: Hlanganani Tutu (ed) Water Quality, InTech, pp 91–109

  • Fiałkowska E, Pajdak-Stós A (2008) The role of Lecane rotifers in activated sludge bulking control. Water Res 42:2483–2490

    Article  CAS  PubMed  Google Scholar 

  • Foissner W, Berger H (1996) A user-friendly guide to the ciliates (Protozoa, Ciliophora) commonly used by hydrobiologists as bioindicators in rivers, lakes, and waste waters, with notes on their ecology. Freshw Biol 35:375–482

    Google Scholar 

  • Fracker SB, Brischle HA (1944) Measuring the local distribution of Ribes. Ecology 25:283–303

    Article  Google Scholar 

  • Fugimura M, dos Reis Flor H, de Melo E et al (2014) Brewery residues as a source of organic carbon in Litopenaeus schmitti white shrimp farms with BFT systems. Aquac Int 23:509–522

    Article  CAS  Google Scholar 

  • Furtado PS, Poersch LH, Wasielesky W Jr (2011) Effect of calcium hydroxide, carbonate and sodium bicarbonate on water quality and zootechnical performance of shrimp Litopenaeus vannamei reared in bio-flocs technology (BFT) systems. Aquaculture 321:130–135

    Article  CAS  Google Scholar 

  • Govedich TR, Bain BA, Moser WE et al (2009) Annelida (Clitellata): Oligochaeta, Branchiobdellida, Hirudinida, and Acanthobdellida. In: Thorp JH, Covich AP (eds) Ecology and classification of north American freshwater invertebrates, 3rd edn. Elsevier, London

    Google Scholar 

  • Hargreaves J (2013) Biofloc production systems for aquaculture. Southern Regional Aquaculture Center publication 4503

  • Holovachov O, De Ley P (2006) Order Plectida. In: Eyualem-Abebe AI, Traunspurger W (eds) Freshwater nematodes: ecology and taxonomy. CABI Publishing, Oxford

    Google Scholar 

  • Ibrahim F, Noll C, Valenti WC (2015) Zooplankton capturing by Nile Tilapia, Oreochromis niloticus (Teleostei: Cichlidae) throughout post-larval development. Zoologia 32:469–475

    Article  Google Scholar 

  • Jeuck A, Arndt H (2013) A short guide to common heterotrophic flagellates of freshwater habitats based on the morphology of living organisms. Protist 164:842–860

    Article  PubMed  Google Scholar 

  • Kim SK, Jo JC, Jang IK (2015) Characterization of bacterial community in biofloc farms. Available via DIALOG. http://media.dhweb.com.s3.amazonaws.com/aes/biofloc/neworleans2015/Kim-Jo-Jang.pdf Cited 28 Oct 2016

  • Krummenauer D, Samocha T, Poersch L, Lara G, Wasielesky W Jr (2014) The reuse of water on the culture of pacific white shrimp Litopenaeus vannamei, in BFT system. J World Aquacult Soc 45:3–14

    Article  CAS  Google Scholar 

  • Lara G, Krummenauer D, Abreu PC et al (2016) The use of different aerators on Litopenaeus vannamei biofloc culture system: effects on water quality shrimp growth and biofloc composition. Aquac Int 25:147–162

    Article  CAS  Google Scholar 

  • Liu W, Luo G, Tan H et al (2016) Effects of sludge retention time on water quality and bioflocs yield, nutritional composition, apparent digestibility coefficients treating recirculating aquaculture system effluent in sequencing batch reactor. Aquac Eng 72–73:58–64

    Article  Google Scholar 

  • Loureiro CK, Wasielesky W Jr, Abreu PC (2012) The use of protozoan, rotifers and nematodes as live food for shrimp raised in BFT system. Atlântica 4:5–12

    Article  Google Scholar 

  • Luna-Pabello VM, Aladro-Lubel A, Durán-de-Bazúa C (1992) Temperature effects on ciliates diversity and abundance in a rotating biological reactor. Bioresour Technol 39:55–60

    Article  CAS  Google Scholar 

  • Manan H, Moh J, Kasan NA et al (2016) Identification of biofloc microscopic composition as the natural bioremediation in zero water exchange of Pacific white shrimp, Penaeus vannamei, culture in closed hatchery system. Appl Water Sci 7:2437–2446

    Article  CAS  Google Scholar 

  • Martínez-Córdova LR, Emerenciano M, Miranda-Baeza A et al (2014) Microbial-based systems for aquaculture of fish and shrimp: an updated review. Rev Aquac 7:131–148

    Article  Google Scholar 

  • Monroy-Dosta M, Lara-Andrade D, Castro-Mejía J et al (2013) Microbiology community composition and abundance associated to biofloc in tilapia aquaculture. Rev Biol Mar Oceanogr 48:511–520

    Article  Google Scholar 

  • Nevejan N, De Schryver P, Wille M et al (2016) Bacteria as food in aquaculture: do they make a difference? Rev Aquac 10:180–212

    Article  Google Scholar 

  • Pandey PK, Bharti V, Kumar K (2014) Biofilm in aquaculture production. Afr J Microbiol Res 8:1434–1443

    Article  CAS  Google Scholar 

  • Patterson DJ, Hedley S (1992) Free-living freshwater protozoa: a color guide. Manson Publishing Ltd., London

    Google Scholar 

  • Pérez-Fuentes J, Hernández-Vergara M, Pérez-Rostro C et al (2016) C:N ratios affect nitrogen removal and production of Nile tilapia Oreochromis niloticus raised in a biofloc system under high density cultivation. Aquaculture 425:247–251

    Article  CAS  Google Scholar 

  • Ray AJ, Lewis BL, Browdy CL, Leffler JW (2010a) Suspended solids removal to improve shrimp (Litopenaeus vannamei) production and an evaluation of a plant-based feed in minimal-exchange superintensive culture systems. Aquaculture 299:89–98

    Article  Google Scholar 

  • Ray AJ, Seaborn G, Leffler JW, Wilde SB, Lawson A, Browdy CL (2010b) Characterization of microbial communities in minimal-exchange, intensive aquaculture systems and the effects of suspended solids management. Aquaculture 310:130–138

    Article  Google Scholar 

  • Wallace RL, Snell TW (2009) Rotifera. In: Thorp JH, Covich AP (eds) Ecology and classification of north American freshwater invertebrates, 3rd edn. Elsevier, London

    Google Scholar 

  • Xu WJ, Morris TC, Samocha TM (2016) Effects of C/N ratio on biofloc development, water quality, and performance of Litopenaeus vannamei juveniles in a biofloc-based, high-density, zero-exchange, outdoor tank system. Aquaculture 453:169–175

    Article  CAS  Google Scholar 

  • Zar J (2010) Biostatistical analysis, 5th edn. Upper Saddle River, Prentice Hall, New Jersey

    Google Scholar 

Download references

Acknowledgements

The authors would also like to thank two anonymous reviewers for their valuable comments on an earlier version of this manuscript.

Funding

A.G.C. is the grant for posgraduate support by the Consejo Nacional de Ciencia y Tecnología de México (CONACYT, grant No. 229713).

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Authors and Affiliations

  1. Laboratorio de Mejoramiento Genético y Producción Acuícola, Instituto Tecnológico de Boca del Río, Km 12 Carretera Veracruz-Córdoba, Boca del Río, 94290, Veracruz, Mexico

    Alfredo Gallardo-Collí, Carlos Iván Pérez-Rostro, Martha Patricia Hernández-Vergara & Ignacio Alejandro Pérez-Legaspi

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  1. Alfredo Gallardo-Collí
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  2. Carlos Iván Pérez-Rostro
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  3. Martha Patricia Hernández-Vergara
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  4. Ignacio Alejandro Pérez-Legaspi
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Correspondence to Carlos Iván Pérez-Rostro.

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Gallardo-Collí, A., Pérez-Rostro, C.I., Hernández-Vergara, M.P. et al. Microeukaryote community and the nutritional composition of the biofloc during Nile tilapia culture in water-reusing biofloc systems. Aquacult Int 27, 381–398 (2019). https://doi.org/10.1007/s10499-018-0335-2

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