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Aquaculture International

, Volume 26, Issue 3, pp 899–911 | Cite as

Effect of nitrite exposure on oxygen-carrying capacity and gene expression of NF-κB/HIF-1α pathway in gill of bighead carp (Aristichthys nobilis)

  • Yan Lin
  • Ling-Hong Miao
  • Wu-Xiao Zhang
  • Wen-Jing Pan
  • Hua-Liang Liang
  • Xian-Ping Ge
  • Yan-Shun Xu
  • Bo Liu
  • Ming-Chun Ren
  • Qun-Lan Zhou
  • Si-Lei Xia
Article
  • 66 Downloads

Abstract

Nitrite (NO2) contamination of water can severely impact the health of aquatic life and is a major concern for commercial aquaculture. In order to study the effect of nitrite on Aristichthys nobilis, we investigated the oxygen-carrying capacity, NF-κB/HIF-1α pathway, and the gill tissue structure under nitrite stress. In the current study, bighead carp (initial weight 180.05 ± 0.092 g) were exposed to nitrite (48.634 mg/L) for 96 h and then for 96 h recovery test. After nitrite exposure for 6 h, hypoxia-inducible factor-1α (HIF-1α) and nuclear factor-κB (NF-κB) mRNA expression increased significantly in the gill of bighead carp (P < 0.05). After nitrite exposure for 12 h, hemoglobin (Hb) and methemoglobin reductase (MHBR) content in blood decreased significantly (P < 0.05); TLR4 mRNA expression increased significantly (P < 0.05). After nitrite exposure for 24 h, methemoglobin (MetHb) content increased significantly (P < 0.05). After recovery test, all the indicators except TLR4 mRNA expression level recovered to initial level. In conclusion, nitrite exposure can affect hemoglobin dynamics, as oxidization of nitrite by hemoglobin results in the reduction of Hb to MetHb leading to hypoxia and nitrite exposure can also result into gill tissue damage. In the face of nitrite exposure, NF-κB and HIF-1α mRNA expression level increased immediately to protect the body from oxidative damage and eased hypoxic condition caused by nitrite. It was also observed that nitrite damage is recoverable in Aristichthys nobilis, but it may be need more than 96 h.

Keywords

Nitrite exposure NF-κB/HIF-1α pathway Aristichthys nobilis 

Notes

Acknowledgements

We would like to thank the post graduate students of Fish Disease and Nutrition Department, Freshwater Fisheries Research Center (FFRC), Chinese Academy of Fishery Sciences (CAFS), PR China, for their help throughout the research period.

Author contributions

Yan Lin, Ling-Hong Miao, and Xian-Ping Ge conceived the idea and designed the project. Wu-Xiao Zhang, Wen-Jing Pan, Hua-Liang Liang, Yan-Shun Xu, Bo Liu, Ming-Chun Ren, Qun-Lan Zhou, and Si-Lei Xia performed the experiments. Yan Lin analyzed the data and wrote the manuscript. All authors have read and approved the final manuscript.

Funding

This work was supported by China Agriculture Research System special project of National Conventional Freshwater Fishery Industry (Grant No.CARS-46), by the National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences (Grant No.2015GBFM25), and by program of Collaborative innovation center of food safety and quality control in Jiangsu Province.

Compliance with ethical standards

All experimental protocols and feeding scheme were approved by the Bioethical Committee of Freshwater Fisheries Research Center (FFRC) of Chinese Academy of Fishery Sciences (CAFS) (BC 2013863, 9/2013).

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Yan Lin
    • 1
  • Ling-Hong Miao
    • 1
    • 2
  • Wu-Xiao Zhang
    • 2
  • Wen-Jing Pan
    • 2
  • Hua-Liang Liang
    • 2
  • Xian-Ping Ge
    • 1
    • 2
  • Yan-Shun Xu
    • 4
  • Bo Liu
    • 1
    • 2
  • Ming-Chun Ren
    • 1
  • Qun-Lan Zhou
    • 1
  • Si-Lei Xia
    • 3
  1. 1.Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture, Freshwater Fisheries Research CenterChinese Academy of Fishery SciencesWuxiPeople’s Republic of China
  2. 2.Wuxi Fisheries CollegeNanjing Agricultural UniversityWuxiChina
  3. 3.College of Animal Science and TechnologyNanjing Agricultural UniversityWuxiChina
  4. 4.Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu ProvinceWuxiChina

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