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Regulation of three isoforms of SOD gene by environmental stresses in citrus red mite, Panonychus citri

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Abstract

Superoxide dismutase (SOD) is a family of enzymes with multiple isoforms that possess antioxidative abilities in response to environmental stresses. Panonychus citri is one of the most important pest mites and has a global distribution. In this study, three distinct isoforms of SOD were cloned from P. citri and identified as cytoplasmic Cu-ZnSOD (PcSOD1), extracellular Cu-ZnSOD (PcSOD2), and mitochondrial MnSOD (PcSOD3). mRNA expression level analysis showed that all three isoforms were up-regulated significantly after exposure to the acaricide abamectin and to UV-B ultraviolet irradiation. In particular, PcSOD3 was up-regulated under almost all environmental stresses tested. The fold change of PcSOD3 expression was significantly higher than those of the two Cu-ZnSOD isoforms. Taken together, the results indicate that abamectin and UV-B can induce transcripts of all three SOD isoforms in P. citri. Furthermore, PcSOD3 seems to play a more important role in P. citri tolerance to oxidative stress.

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Acknowledgments

This research was supported in part by the National Natural Science Foundation (31171851), the Special Fund for Agro-scientific Research in the Public Interest (201103020), the Program for Innovative Research Team in Universities (IRT0976), and the earmarked fund for the Modern Agro-industry (Citrus) Technology Research System of China to JJW, and by the Fundamental Research Funds for the Central Universities (XDJK2013A017) of China to WD.

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  1. Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, 400715, People’s Republic of China

    Ying-Cai Feng, Chong-Yu Liao, Wen-Kai Xia, Xuan-Zhao Jiang, Feng Shang, Guo-Rui Yuan & Jin-Jun Wang

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Correspondence to Jin-Jun Wang.

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Feng, YC., Liao, CY., Xia, WK. et al. Regulation of three isoforms of SOD gene by environmental stresses in citrus red mite, Panonychus citri . Exp Appl Acarol 67, 49–63 (2015). https://doi.org/10.1007/s10493-015-9930-3

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