Spirosoma taeanense sp. nov., a radiation resistant bacterium isolated from a coastal sand dune

Abstract

An aerobic, Gram-negative, non-motile, non-spore-forming, rod-shaped, and pale yellow-colored bacterial strain, designated TS118T, was isolated from a sand sample obtained from a coastal sand dune after exposure to 3 kGy of gamma radiation. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate was a member of the genus Spirosoma and most closely related to Spirosoma metallicum PR1014kT (95.1% similarity). The genome of strain TS118T is constituted by one chromosome (5,691,492 bp) and one plasmid (28,440 bp) and has a G + C content of 52.7%. The genome contains 4641 protein coding sequences (CDSs), 38 tRNAs, and 11 rRNAs. The predominant fatty acids of strain TS118T were C16:1 ω5c, iso-C15:0, C16:0, summed feature 3 (C16:1 ω6c and/or C16:1 ω7c), and iso-C17:0 3-OH. The major polar lipids were phosphatidylethanolamine, an unidentified amino lipid and an unidentified aminophospholipid. The main respiratory quinone was menaquinone-7 (MK-7). The novel strain showed resistance to gamma radiation with a D10 value (i.e., the dose required to reduce the bacterial population by tenfold) of 4.3 kGy. Based on the phylogenetic, physiological, and chemotaxonomic characteristics, strain TS118T represents a novel species, for which the name Spirosoma taeanense sp. nov. is proposed. The type strain is TS118T (=KCTC 72898T =JCM 34024T).

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Availability of data and material

The complete genome sequence of strain TS118T generated during the current study has been deposited in GenBank with the accession codes, https://ncbi.nlm.nih.gov/nuccore/CP053435.1 and https://ncbi.nlm.nih.gov/nuccore/CP053436.1. The 16S rRNA gene sequence also is available in the GenBank repository, https://ncbi.nlm.nih.gov/nuccore/ MN911322.1.

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Funding

This research was supported by the Nuclear R&D program of the Ministry of Science and ICT (MSIT), Republic of Korea.

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Conceptualization, funding acquisition, and supervision: SL; Resources: M-KK; Investigation validation, and visualization: JHL, J-HJ, HNC; Data curation and formal analysis: JHL, J-HJ, CNS; Writing-original draft: JHL; Writing-review and editing: SL.

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Correspondence to Sangyong Lim.

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Lee, J.H., Jung, JH., Kim, MK. et al. Spirosoma taeanense sp. nov., a radiation resistant bacterium isolated from a coastal sand dune. Antonie van Leeuwenhoek 114, 151–159 (2021). https://doi.org/10.1007/s10482-020-01508-0

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Keywords

  • Spirosoma
  • Coastal sand dune
  • Taxonomy
  • Bacteroidetes
  • Gamma radiation
  • Phylogenetic analysis