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Paenibacillus bryophyllum sp. nov., a nitrogen-fixing species isolated from Bryophyllum pinnatum

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Abstract

A nitrogen-fixing, endospore-forming bacterium, designated strain L201T was isolated from the leaves of Bryophyllum pinnatum growing in South China Agricultural University. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain L201T is affiliated with the genus Paenibacillus, and closely related to Paenibacillus albidus Q4-3T (97.4%), Paenibacillus odorifer DSM 15391T (97.3%) and Paenibacillus borealis DSM 13188T (97.2%). The main fatty acids components was anteiso-C15:0 (48.1%). The predominant isoprenoid quinone was MK-7. The major polar lipids were found to be diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The G+C content of strain L201T was 43.9%. DNA–DNA relatedness between L201T and the reference strain was 29.8%. Biological and biochemical tests, protein patterns, genomic DNA fingerprinting and comparison of cellular fatty acids distinguished strain L201T from the closely related Paenibacillus species. Based on these data, the novel species Paenibacillus bryophyllum sp. nov. is proposed, with the type strain L201T(= KCTC 33951 T = GDMCC 1.1251 T).

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31370052). We thank Dr. Meiyang Duan for helping with fatty acid analysis.

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Authors and Affiliations

  1. Guangdong Provincial Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, 483 Wushan Road, Guangzhou, 510642, People’s Republic of China

    Lihui Liu, Tao Yuan, Meiyan Yang & Zhiyuan Tan

  2. College of Resources and Environment, South China Agricultural University, Guangzhou, 510642, People’s Republic of China

    Fei Yang, Ziwei Liu & Guixiang Peng

Authors
  1. Lihui Liu
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  2. Tao Yuan
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  3. Fei Yang
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  4. Ziwei Liu
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  5. Meiyan Yang
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  6. Guixiang Peng
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  7. Zhiyuan Tan
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Correspondence to Zhiyuan Tan.

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Additional information

The GenBank accession numbers for 16S rRNA gene sequences of strain L201T is MF040761.

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Liu, L., Yuan, T., Yang, F. et al. Paenibacillus bryophyllum sp. nov., a nitrogen-fixing species isolated from Bryophyllum pinnatum. Antonie van Leeuwenhoek 111, 2267–2273 (2018). https://doi.org/10.1007/s10482-018-1117-6

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  • DOI: https://doi.org/10.1007/s10482-018-1117-6

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