Nonomuraea insulae sp. nov., isolated from forest soil
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Strain H2R21T, a novel actinobacterium, isolated from a forest soil sample collected from Heybeliada, Istanbul, Turkey, and a polyphasic approach was used for characterisation of the strain. Chemotaxonomic and morphological characterisation of strain H2R21T indicated that it belongs to the genus Nonomuraea. 16S rRNA gene sequence similarity showed that the strain is closely related to Nonomuraea purpurea 1SM4-01T (99.1%) and Nonomuraea solani CGMCC 4.7037T (98.4%). DNA–DNA relatedness values were found to be lower than 70% between the isolate and its phylogenetic neighbours N. purpurea 1SM4-01T, N. solani CGMCC 4.7037T and Nonomuraea rhizophila YIM 67092T. The whole cell hydrolysates of strain H2R21T were found to contain meso-diaminopimelic acid as the diagnostic diamino acid and glucose, madurose, mannose and ribose as the cell sugars. The polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, dihydroxy-phosphatidylethanolamine, phosphatidylinositol, glycophosphatidylinositol, two glycophospholipids and two unidentified lipids. The predominant menaquinones were identified as MK-9(H4) and MK-9(H6). The major fatty acids were found to be iso-C16:0, iso-C16:0 2OH and C17:0 10-methyl. On the basis of DNA–DNA relatedness data and some phenotypic characteristics, it is evident that strain H2R21T can be distinguished from the closely related species in the genus Nonomuraea. Thus, it is concluded that strain H2R21T represents a novel species of the genus Nonomuraea, for which the name Nonomuraea insulae sp. nov. is proposed. The type strain is H2R21T (= DSM 102915T = CGMCC 4.7338T = KCTC 39769T).
KeywordsActinobacteria Nonomuraea insulae sp. nov. Polyphasic taxonomy 16S rRNA gene
This research was supported by Ondokuz Mayis University (OMU), project no. PYO.FEN.1904.13.004. We gratefully acknowledges Dr. Kannika Duangmal from Kasetsart University in Thailand for help to us in obtaining one of the type species.
Conflict of interest
The authors declare that they have no conflict of interest.
- Collins MD (1985) Isoprenoid quinone analysis in bacteria classification and identification. In: Goodfellow M, Minnikin DE (eds) Chemical methods in bacterial systematics. Academic Press, London, pp 267–287Google Scholar
- Kämpfer P (2012) Genus VI. Nonomuraea. In: Whitman WB, Goodfellow M, Kämpfer P, Busse H-J, Trujillo ME, Ludwig W, Suzuki KI, Parte A (eds) Bergey’s manual of systematic bacteriology. The Actinobacteria, vol 5, 2nd edn. Springer, New York, pp 1844–1861Google Scholar
- Kelly KL (1964) Inter-society color council-national bureau of standards color-name charts illustrated with centroid colors. US Government Printing Office, Washington, DCGoogle Scholar
- Nash P, Krent MM (1991) Culture media. In: Balows A, Hauser WJ, Herrmann KL, Isenberg HD, Shadomy HJ (eds) Manual of clinical microbiology, 3rd edn. American Society for Microbiology, Washington, DC, pp 1268–1270Google Scholar
- Saitou N, Nei M (1987) The neighbor-joining method. A new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425Google Scholar
- Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. Technical Note 101. MIDI Inc: NewarkGoogle Scholar
- Waksman SA (1961) The actinomycetes, classification, identification and description of genera and species, vol 2. Williams & Wilkins, BaltimoreGoogle Scholar
- Waksman SA (1967) The actinomycetes. A summary of current knowledge. Ronald Press, New YorkGoogle Scholar