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Nonomuraea insulae sp. nov., isolated from forest soil

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Abstract

Strain H2R21T, a novel actinobacterium, isolated from a forest soil sample collected from Heybeliada, Istanbul, Turkey, and a polyphasic approach was used for characterisation of the strain. Chemotaxonomic and morphological characterisation of strain H2R21T indicated that it belongs to the genus Nonomuraea. 16S rRNA gene sequence similarity showed that the strain is closely related to Nonomuraea purpurea 1SM4-01T (99.1%) and Nonomuraea solani CGMCC 4.7037T (98.4%). DNA–DNA relatedness values were found to be lower than 70% between the isolate and its phylogenetic neighbours N. purpurea 1SM4-01T, N. solani CGMCC 4.7037T and Nonomuraea rhizophila YIM 67092T. The whole cell hydrolysates of strain H2R21T were found to contain meso-diaminopimelic acid as the diagnostic diamino acid and glucose, madurose, mannose and ribose as the cell sugars. The polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, dihydroxy-phosphatidylethanolamine, phosphatidylinositol, glycophosphatidylinositol, two glycophospholipids and two unidentified lipids. The predominant menaquinones were identified as MK-9(H4) and MK-9(H6). The major fatty acids were found to be iso-C16:0, iso-C16:0 2OH and C17:0 10-methyl. On the basis of DNA–DNA relatedness data and some phenotypic characteristics, it is evident that strain H2R21T can be distinguished from the closely related species in the genus Nonomuraea. Thus, it is concluded that strain H2R21T represents a novel species of the genus Nonomuraea, for which the name Nonomuraea insulae sp. nov. is proposed. The type strain is H2R21T (= DSM 102915T = CGMCC 4.7338T = KCTC 39769T).

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Acknowledgements

This research was supported by Ondokuz Mayis University (OMU), project no. PYO.FEN.1904.13.004. We gratefully acknowledges Dr. Kannika Duangmal from Kasetsart University in Thailand for help to us in obtaining one of the type species.

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Authors and Affiliations

  1. Department of Nursing, School of Health, Ahi Evran University, 40100, Kırşehir, Turkey

    Salih Saricaoglu

  2. Department of Biology, Faculty of Science and Arts, Ondokuz Mayis University, 55139, Samsun, Turkey

    Salih Saricaoglu, Hayrettin Saygin, Kamil Isik & Nevzat Sahin

  3. School of Natural and Environmental Sciences, Newcastle University, Ridley Building 2, Newcastle upon Tyne, NE1 7RU, UK

    Imen Nouioui & Hans-Peter Klenk

  4. Department of Molecular Biology and Genetics, Faculty of Sciences and Arts, Ondokuz Mayis University, 55139, Samsun, Turkey

    Hilal Ay

  5. Department of Molecular Biology and Genetics, Faculty of Science, Karadeniz Technical University, 61080, Trabzon, Turkey

    Kadriye Inan Bektas

  6. Department of Biology, Faculty of Science, Anadolu University, 26470, Eskisehir, Turkey

    Kiymet Guven

  7. Division of Science Education, Department of Mathematics and Science Education, Gazi University, 06500, Ankara, Turkey

    Demet Cetin

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Correspondence to Nevzat Sahin.

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Saricaoglu, S., Nouioui, I., Ay, H. et al. Nonomuraea insulae sp. nov., isolated from forest soil. Antonie van Leeuwenhoek 111, 2051–2059 (2018). https://doi.org/10.1007/s10482-018-1097-6

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