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Metschnikowia mating genomics

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Abstract

Genes involved in mating type determination and recognition were examined in Metschnikowia and related species, to gather insights on factors affecting mating compatibility patterns among haplontic, heterothallic yeast species of the genus. We confirmed the universality of the special mating locus organisation found in Clavispora lusitaniae across and exclusive to the family Metschnikowiaceae (i.e., Metschnikowia and Clavispora). Timing of the divergence between idiomorphs was confirmed to coincide with the origin of the larger (CUG-ser) clade comprising the Debaryomycetaceae and the Metschnikowiaceae, exclusive of Cephaloascus fragrans. The sequence of the a mating pheromone is highly conserved within the large-spored Metschnikowia species, including Metschnikowia orientalis and Metschnikowia hawaiiana, but not Metschnikowia drosophilae or Metschnikowia torresii, which have a pattern of their own, as do other clades in the genus. In contrast, variation in α pheromones shows a more continuous, although imperfect correlation with phylogenetic distance as well as with in vivo mating compatibility.

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Acknowledgements

Funding from the Natural Science and Engineering Research Council of Canada is gratefully acknowledged. Thanks are extended to K. Bensch for her assistance in obtaining the MycoBank numbers and to A. Spera for early contributions to exploration of the mating type locus.

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Authors and Affiliations

  1. Department of Biology, University of Western Ontario, London, ON, N6A 5B7, Canada

    Dong Kyung Lee & Marc-André Lachance

  2. School of Environmental Sciences, University of Guelph, Guelph, ON, N1G 2W1, Canada

    Tom Hsiang

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  1. Dong Kyung Lee
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Correspondence to Marc-André Lachance.

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Lee, D.K., Hsiang, T. & Lachance, MA. Metschnikowia mating genomics. Antonie van Leeuwenhoek 111, 1935–1953 (2018). https://doi.org/10.1007/s10482-018-1084-y

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