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Antonie van Leeuwenhoek

, Volume 111, Issue 10, pp 1845–1853 | Cite as

Hoeflea prorocentri sp. nov., isolated from a culture of the marine dinoflagellate Prorocentrum mexicanum PM01

  • Qiao Yang
  • Zhi-Wei Jiang
  • Cheng-Hui Huang
  • Ruo-Nan Zhang
  • Ling-Zhi Li
  • Guang Yang
  • Li-Juan Feng
  • Guang-Feng Yang
  • Heng Zhang
  • Xiao-Ling Zhang
  • Jun Mu
Original Paper

Abstract

A Gram-stain negative, aerobic, rod-shaped, non-motile, yellow-pigmented and non-spore-forming bacterial strain, designated PM5-8T, was isolated from a culture of a marine toxigenic dinoflagellate Prorocentrum mexicanum PM01. Strain PM5-8T grew at 15–35 °C (optimum, 25–30 °C) and pH 6–11 (optimum, 7.5–8). Cells required at least 1.5% (w/v) NaCl for growth, and can tolerate up to 7.0% with the optimum of 4%. Phylogenetic analysis based on 16S rRNA gene sequence revealed that the strain PM5-8T is closely related to members of the genus Hoeflea, with high sequence similarities with Hoeflea halophila JG120-1T (97.06%) and Hoeflea alexandrii AM1V30T (97.01%). DNA–DNA hybridization values between the isolate and other type strains of recognized species of the genus Hoeflea were between 11.8 and 25.2%, which is far below the value of 70% threshold for species delineation. The DNA G + C content was 50.3 mol%. The predominant cellular fatty acids of the strain were identified as summed feature 8 (C16:1 ω7c and/or C16:1 ω6c; 51.5%), C18:1 ω7c 11-methyl (20.7%), C16:0 (17.2%) and C18:0 (5.7%). The major respiratory quinone was Q-10. Polar lipids profiles contained phosphatidylcholine, phosphatidylglycerol, sulfoquinovosyl diacylglycerol, phosphatidylmono- methylethanolamine, phosphatidylethanolamine and four unidentified lipids. On the basis of the polyphasic taxonomic data presented, strain PM5-8T (= CCTCC AB 2016294T = KCTC 62490T) represents a novel species of the genus Hoeflea, for which the name Hoeflea prorocentri sp. nov. is proposed.

Keywords

Hoeflea prorocentri sp. nov. Novel species Prorocentrum mexicanum Algal-associated bacterium 

Notes

Acknowledgements

This work was supported by Talent Introduction Foundation of Zhejiang Ocean University (for Qiao Y.), the National Natural Science Foundation of China (41206093 and 31470540), the Zhejiang Provincial Natural Science Foundation of China (LY18D060007), Scientific Instrument and Chemical Reagents Project of Shanghai Science and Technology Committee (15142201600), and Municipal Public Welfare Project of Zhoushan (2017C32083). The authors also sincerely thank Prof. Hong-Nong Chou at Institute of Fisheries Science, National Taiwan University, for kindly providing Prorocentrum mexicanum PM01.

Compliance with ethical standards

Conflict of interest

All the authors declared that they have no conflict of interest.

Human and animal rights

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

10482_2018_1074_MOESM1_ESM.docx (497 kb)
Supplementary material 1 (DOCX 496 kb)

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Qiao Yang
    • 1
  • Zhi-Wei Jiang
    • 1
  • Cheng-Hui Huang
    • 1
  • Ruo-Nan Zhang
    • 2
  • Ling-Zhi Li
    • 3
  • Guang Yang
    • 1
  • Li-Juan Feng
    • 1
  • Guang-Feng Yang
    • 4
  • Heng Zhang
    • 3
  • Xiao-Ling Zhang
    • 1
  • Jun Mu
    • 1
  1. 1.Laboratory of Marine Environment and Ecology, College of Marine Science and TechnologyZhejiang Ocean UniversityZhoushanPeople’s Republic of China
  2. 2.Center of Research in Life Sciences and Environmental SciencesHarbin University of CommerceHarbinPeople’s Republic of China
  3. 3.East China Sea Fisheries Research Institute, Chinese Academy of Fishery SciencesShanghaiPeople’s Republic of China
  4. 4.Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of SciencesQingdaoPeople’s Republic of China

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