Streptomyces urticae sp. nov., isolated from rhizosphere soil of Urtica urens L.
Two novel Gram-stain positive, spore-forming, aerobic actinomycetes, designated NEAU-PCY-1T and NEAU-PCY-2, were isolated from rhizosphere soil of Urtica urens L. collected from Anshan, Liaoning Province, northeast China. The 16S rRNA gene sequence analysis showed that strains NEAU-PCY-1T and NEAU-PCY-2 exhibited 99.8% similarity with each other and are closely related to Streptomyces abietis DSM 42080T (98.2, 98.3%) and Streptomyces fildesensis DSM 41987T (98.0, 98.1%). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the two strains formed a cluster with these two closely related species. Moreover, DNA–DNA hybridization results and some phenotypic, physiological and biochemical properties differentiated the two strains from their close relatives in the genus Streptomyces. Based on a polyphasic taxonomy study, strains NEAU-PCY-1T and NEAU-PCY-2 are considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces urticae sp. nov. is proposed, with NEAU-PCY-1T (= DSM 105115T = CCTCC AA 2017015T) as the type strain.
KeywordsStreptomyces urticae sp. nov. Polyphasic taxonomy 16S rRNA gene Rhizosphere soil of Urtica urens L.
This work was supported in part by grants from the National Natural Science Foundation of China (Nos. 31572070, 31672092 and 31471832), Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (CSCP). We are grateful to Prof. Aharon Oren for helpful advice on the specific epithet.
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Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants and/or animals performed by any of the authors. The formal consent is not required in this study.
- Collins MD (1985) Chemical methods in bacterial systematics. In: Goodfellow M, Minnikin DE (eds) Isoprenoid quinone analyses in bacterial classification and identification. Academic Press, London, pp 267–284Google Scholar
- Hatano K, Nishii T, Kasai H (2003) Taxonomic re-evaluation of whorl-forming Streptomyces (formerly Streptoverticillium) species by using phenotypes, DNA-DNA hybridization and sequences of gyr B, and proposal of Streptomyces luteireticuli (ex Katoh and Arai 1957) corrig., sp. nov., nom. rev. Int J Syst Evol Microbiol 53:1519–1529CrossRefGoogle Scholar
- Kelly KL (1964) Inter-society colour council-national bureau of standards colour-name charts illustrated with centroid colours published in USGoogle Scholar
- Kim KO, Shin KS, Kim MN, Shin KS, Labeda DP, Han JH, Kim SB (2012) Reassessment of the status of streptomyces setonii and reclassification of streptomyces fimicarius as a later synonym of streptomyces setonii and streptomyces albovinaceus as a later synonym of streptomyces globisporus based on combined 16S rRNA/gyrB gene sequence analysis. Int J Syst Evol Microbiol 62(Pt 12):2978CrossRefGoogle Scholar
- Lechevalier HA, Lechevalier MP (1970a) A critical evaluation of the genera of aerobic actinomycetes. In: Prauser H (ed) The actinomycetes. Gustav Fischer, Jena, pp 393–405Google Scholar
- Lechevalier MP, Lechevalier HA (1980) The chemotaxonomy of actinomycetes. In: Dietz A, Thayer DW (eds) Actinomycete taxonomy special publication, vol 6. Society of Industrial Microbiology, Arlington, pp 227–291Google Scholar
- Mzid M, Ghlissi Z, Salem MB, Khedir SB, Chaabouni K, Ayedi F, Sahnoun Z, Hakim A, Rebai T (2017b) Chemoprotective role of ethanol extract of Urtica urens L. against the toxicity of imidacloprid on endocrine disruption and ovarian morphometric in female rats, GC/MS analysis. Biomed Pharmacother 97:518–527CrossRefGoogle Scholar
- Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425Google Scholar
- Smibert RM, Krieg NR (1994) Phenotypic characterisation. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington DC, pp 607–654Google Scholar
- Waksman SA (1961) The Actinomycetes, vol. 2, Classification, identification and descriptions of genera and species. Baltimore: Williams and WilkinsGoogle Scholar
- Waksman SA (1967) The actinomycetes a summary of current knowledge. Ronald Press, New YorkGoogle Scholar
- Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE (1987) International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464CrossRefGoogle Scholar
- Williams ST, Goodfellow M, Alderson G (1989) Genus Streptomyces Waksman and Henrici 1943, 339AL. In: Williams ST, Sharpe ME, Holt JG (eds) Bergey’s manual of systematic bacteriology, vol 4. Williams and Wilkins, Baltimore, pp 2452–2492Google Scholar
- Wu C, Lu X, Qin M, Wang Y, Ruan J (1989) Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16:176–178Google Scholar