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Phenylobacterium terrae sp. nov., isolated from a soil sample of Khyber-Pakhtun-Khwa, Pakistan

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Abstract

A Gram-stain negative, aerobic, motile, non-spore-forming and rod-shaped bacterial strain, designated YIM 730227T, was isolated from a soil sample, collected from Karak district, Khyber-Pakhtun-Khwa, Pakistan. The bacterium was characterized using a polyphasic taxonomic approach. Pairwise comparison of the 16S rRNA gene sequences showed that strain YIM 730227T is closely related to Phenylobacterium lituiforme FaiI3T (97.5% sequence similarity), Phenylobacterium muchangponense A8T (97.4%), Phenylobacterium panacis DCY109T (97.1%), Phenylobacterium immobile ET (97.1%) and Phenylobacterium composti 4T-6T (97.0%), while also sharing 98.0% sequence similarity with Phenylobacterium hankyongense HKS-05T after NCBI blast, showing it represents a member of the family Caulobacteraceae. The major respiratory quinone was Q-10 and the major fatty acids were C16:0, summed feature 8 (comprising C18:1ω7c and/or C18:1ω6c), C18:1ω7c 11-methyl and C17:0. The polar lipids were phosphatidylglycerol, unidentified glycolipids, phospholipid and unidentified lipid. The G + C content of the genomic DNA was 68.2 mol%. The DNA–DNA relatedness values of strain YIM 730227T with P. hankyongense HKS-05T, P. lituiforme FaiI3T, P. muchangponense A8T, P. panacis DCY109T, P. immobile ET and P. composti 4T-6T were 31.3 ± 0.6, 26.1 ± 0.2, 24.3 ± 0.1, 21.8 ± 0.9, 19.8 ± 0.6 and 18.2 ± 1.1%, respectively, values lower than 70%. Besides the morphological and chemotaxonomic characteristics, phylogenetic analyses of 16S rRNA gene sequences and the biochemical characteristics indicated that the strain YIM 730227T represents a novel member of the genus Phenylobacterium, for which the name Phenylobacterium terrae sp. nov. (type strain YIM 730227T = KCTC62324T = CGMCC 1.16326T) is proposed.

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Acknowledgements

We are grateful to Dr Rüdiger Pukall (DSMZ, Germany) and Professor Jung-Sook Lee (KCTC, Korea) for kindly providing the reference type strains. This work was supported by Science and Technology Infrastructure work project of Ministry of Science & Technology (No. 2015FY110100) and National Natural Science Foundation of China (No. 31470139). W-J Li was also supported by Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme (2014).

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Author notes

  1. Inam Ullah Khan, Neeli Habib and Min Xiao have contributed equally to this work.

Authors and Affiliations

  1. Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, 650091, People’s Republic of China

    Inam Ullah Khan, Neeli Habib, Xiao-Yang Zhi & Wen-Jun Li

  2. State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Min Xiao & Wen-Jun Li

  3. Department of Biological Sciences, Gomal University, Dera Ismail Khan, KPK, Pakistan

    Inam Ullah Khan

  4. Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People’s Republic of China

    Xing Huang

  5. The Applied Plant Genomics Laboratory of Crop Genomics and Bioinformatics Centre, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People’s Republic of China

    Nasr Ullah Khan

  6. Department of Biotechnology, Hankyong National University, 327 Chungang-no, Anseong-si, Kyonggi-do, 456-749, Republic of Korea

    Wan-Taek Im

  7. Institute of Microbial Culture Collection of Pakistan (IMCCP), National Agricultural Research Centre (NARC), Park Road, Islamabad, 45500, Pakistan

    Iftikhar Ahmed

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  1. Inam Ullah Khan
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Correspondence to Xiao-Yang Zhi or Wen-Jun Li.

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It is the original work of the authors. The work described has not been submitted elsewhere for publication, in whole or in part, and all authors listed carry out the data analysis and manuscript writing and ‘‘This article does not contain any studies with human participants or animals performed by any of the authors’’. Moreover, all authors read and approved the final manuscript.

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Khan, I.U., Habib, N., Xiao, M. et al. Phenylobacterium terrae sp. nov., isolated from a soil sample of Khyber-Pakhtun-Khwa, Pakistan. Antonie van Leeuwenhoek 111, 1767–1775 (2018). https://doi.org/10.1007/s10482-018-1064-2

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